中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (2): 132-134.

• 论著 • 上一篇    下一篇

黄芩苷对中波紫外线诱导后BALB/c小鼠表皮光产物形成的干预作用

骆丹 周炳荣   

  1. 南京市南京医科大学附属第一医院皮肤科 南京医科大学第一附属医院
  • 收稿日期:2008-03-10 修回日期:2008-03-26 出版日期:2009-02-15 发布日期:2009-02-15
  • 通讯作者: 骆丹 E-mail:daniluo2005@yahoo.com.cn

Interference by baicalin in the formation of photoproduct in epidermal cells from BALB/c mice irradiated with ultroviolet B

  • Received:2008-03-10 Revised:2008-03-26 Online:2009-02-15 Published:2009-02-15

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摘要：

目的 观察中波紫外线（UVB）诱导BALB/c小鼠皮肤表皮细胞光产物的形成和清除情况，以及黄芩苷的干预作用。方法 将黄芩苷（1 mg/cm2）连续3 d外用于BALB/c小鼠表皮24 h后，采用免疫组织化学法及免疫印迹法检测180 mJ/cm2 UVB辐射后1 h、24 h和48 h小鼠表皮内环丁烷嘧啶二聚体（CPD）的产生和清除情况。结果 CPD仅在接受UVB辐射的小鼠皮肤内出现。相对于UVB辐射后1 h光产物的平均值，UVB辐射组1 h、24 h和48 h，CPD的信号强度分别为（100 ± 5.22）％、（75.34 ± 8.22）％和（42.11 ± 3.24）％；经过黄芩苷处理后的小鼠接受UVB辐射后1 h、24 h和48 h，CPD的信号强度分别为（81.45 ± 5.22）%、（32.14 ± 6.33）%和（5.21 ± 3.15）%；而丙酮处理后的小鼠接受UVB辐射后1 h、24 h和48 h，CPD的信号强度分别为（106 ± 8.21）%、（70.23 ± 4.13）%和（41.22 ± 4.21）%。结论 外用黄芩苷能抑制UVB辐射诱导光产物的形成，并在48 h内加速光产物的清除。黄芩苷是一种有效的紫外线防护剂。

关键词: 黄芩苷;环丁烷嘧啶二聚体;中波紫外线

Abstract:

Objective To investigate the formation and elimination of photoproduct in epidermal cells from BALB/c mice irradiated with ultroviolet B, and to observe the interference by baicalin in it. Methods BALB/c mice were randomized into 6 groups, i.e., blank control group receiving no exposure or protection, baicalin group receiving protection with baicalin, acetone group receiving acetone pretreatment, UVB group receiving UVB irradiation but no protection, UVB + baicalin group receiving UVB irradiation and protection with baicalin, UVB + acetone group receiving acetone pretreatment and UVB irradiation. Baicalin was applied at 1 mg/cm2 on the back of mice for 3 days in baicalin group and UVB + baicalin group. Twenty hours after the last application, UVB irradiation of 180 mJ/cm2 was given to mice in UVB group and UVB + baicalin group. Skin specimens were obtained from the tested sites at 1, 24, and 48 hours, respectively, after the irradiation. Cyclobutane pyrimidine dimers （CPD） was detected in the specimens with immunohistochemical staining and Southwestern dot blotting. Results CPD was observed only in irradiated mice. The relative content of CPD in epidermal cells 1, 24 and 48 hours after the irradiation was （100 ± 5.22)％, （75.34 ± 8.22）％ and （42.11 ± 3.24）％, respectively, in UVB group, （81.45 ± 5.22）％, （32.14 ± 6.33）％ and （5.21 ± 3.15）％ respectively, in UVB+baicalin group, （106 ± 8.21）％, （70.23 ± 4.13）％ and （41.22 ± 4.21）％, respectively, in UVB + acetone group. A significant difference was observed in the relative content of CPD between UVB group and UVB + baicalin group at 1, 24 and 48 hours after the irradiation （P < 0.05, 0.01, 0.01, respectively）. Conclusions Taken together, these results suggest that topical baicalin application mitigates DNA photo-damage. Baicalin is therefore a promising protective substance against UVB radiation.

Key words: Baicalin;cyclobutane pyrimidine dimers;ultraviolet B