中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (2): 119-121.

• 论著 • 上一篇    下一篇

Fascin-1蛋白在基底细胞癌及鳞状细胞癌中的表达

金城 朱小红 赵志国 李明 华海康 杨莉佳   

  1. 南京医科大学附属无锡第二医院皮肤科 江苏省无锡市第二人民医院皮肤科 镇江市第一人民医院 （江苏大学附属人民医院）皮肤科 无锡市南京医科大学附属无锡第二医院皮肤科 无锡市第二人民医院 江苏省无锡市第二人民医院皮肤科
  • 收稿日期:2008-02-26 修回日期:2008-04-02 出版日期:2009-02-15 发布日期:2009-02-15
  • 通讯作者: 金城 E-mail:jincheng1013@yahoo.com.cn

Expression of fascin-1 protein in basal cell carcinoma and squamous cell carcinoma

  • Received:2008-02-26 Revised:2008-04-02 Online:2009-02-15 Published:2009-02-15

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摘要：

目的 探讨fascin-1蛋白与基底细胞癌及鳞状细胞癌局部侵袭的相关性。方法 免疫组化方法对10例正常皮肤组织、13例皮肤基底细胞癌（8例结节型、5例浅表型）、24例皮肤鳞状细胞癌（11例原位鳞状细胞癌，13例侵袭性鳞状细胞癌均无转移）进行fascin-1染色，计算机图像分析系统进行观察及定量分析。结果 Fascin-1蛋白在原位鳞状细胞癌（平均光密度0.1152 ± 0.04574）、侵袭性鳞状细胞癌（平均光密度0.1257 ± 0.03096）中的表达明显高于正常上皮组织（平均光密度0.0293 ± 0.00981，P ＜ 0.05）。在结节型基底细胞癌（平均光密度0.0808 ± 0.05642）、浅表型基底细胞癌（平均光密度0.0806 ± 0.04346）中表达差异无统计学意义，但明显高于正常上皮组织（平均光密度0.0293 ± 0.00981，P ＜ 0.05）。结论 在基底细胞癌及鳞状细胞癌中，fascin-1蛋白表达上调可能与肿瘤的局部侵袭相关。

关键词: fascin-1;皮肤肿瘤;侵袭;免疫组化;图像分析

Abstract:

Objective To investigate the relationship of fascin-1 protein expression with the metastasis of basal cell carcinoma and squamous cell carcinoma. Methods Skin specimens were obtained from 10 normal human controls, 13 patients with basal cell carcinoma （8 nodular variant and 5 superficial variant） and 24 patients with SCC （11 SCC in situ and 13 invasive SCC）. Immunohistochemical staining was performed to analyze the expression of fascin-1 protein. The staining results were quantitatively assessed with computer image analysis system （Image-pro Plus 6.0）. Results The optical density of fascin-1 averaged 0.1152 ± 0.04574 in SCC in situ, 0.1257 ± 0.03096 in invasive SCC, and 0.0293 ± 0.00981 in normal controls; the expression of fascin-1 was significantly higher in SCC tissue than in normal control skin （both P < 0.05）. Increased optical density was also observed for fascin-1 in nodular variant of SCC （0.0808 ± 0.05642） and superficial variant of SCC （0.0806 ± 0.04346） compared with the normal controls, whereas no statistical difference was observed between nodular and superficial variant of SCC. Conclusion In BCC and SCC, there is an over expression of fascin-1, which may be linked to the local invasion of carcinoma.

Key words: fascin-1;skin neoplasia;invation;immunohistochemistry;image analysis