关键词: 女贞子;成黑素细胞;迁移

Abstract:

Objective To study the effects of Fructus ligustri lucidi and its monomers, tyrosol and oleanolic acid, on the migration of mouse melanoblast cell line （NCCmelb4M5）. Methods Cultured NCCmelb4M5 cells were treated with Fructus ligustri lucidi （0.0625, 0.125, 0.25, 0.5, 2 mg/mL）, tyrosol （0.02, 0.04, 0.08, 0.16, 0.8 mg/mL） and oleanolic acid （0.0625, 0.125, 0.25, 0.5, 2.5 mg/mL）, respectively, for 48 hours followed by the detection of cell proliferation with MTT assay. The working concentration of the three drugs was determined according to the results of MTT assay. Scratch and transwell assays were performed to observe the effect of Fructus ligustri lucidi and its monomers at working concentration on the migration of NCCmelb4M5 cells. Results Based on the results of MTT assay, the working concentration of Fructus ligustri lucidi, tyrosol and oleanolic acid was determined at 0.125 mg/mL, 0.08 mg/mL and 0.0625 mg/mL respectively, and at these concentrations, these drugs exhibited a cytotoxity lower than that of absolute alcohol with no obvious stimulation of cell proliferation. Scratch and transwell assay revealed a promoting effect of both Fructus ligustri lucidi and tyrosol on melanoblast migration （P < 0.05）, while oleanolic acid had little effect on melanoblast migration. Conclusions The extract of Fructus ligustri lucidi has a significant stimulatory effect on the migration of mouse melanoblasts, and tyrosol may be an active component of Fructus ligustri lucidi associated with confirmative effect on migration of mouse melanoblasts.

Key words: Fructus Ligustri Lucidi;melanoblast;migration