中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (12): 824-827.

• 论著 • 上一篇    下一篇

汉族寻常性银屑病患者IFN-γ受体2基因多态性研究

顾宁琰1,顾恒2,姚煦3,周武庆4,崔盘根3,陈敏3   

  1. 1. 中国医学科学院北京协和医学院皮肤病研究所
    2. 中国医学科学院皮肤病医院（研究所）
    3. 南京 中国医学科学院北京协和医学院皮肤病研究所
    4. 南京医科院皮研所
  • 收稿日期:2009-03-06 修回日期:2009-04-22 出版日期:2009-12-15 发布日期:2012-03-27
  • 通讯作者: 顾宁琰 E-mail:ningyangu@139.com
  • 基金资助:

    江苏省“六大高峰人才”基金

Polymorphism of interferon-γ receptor 2 gene in patients with psoriasis vulgaris in Chinese Hans

  • Received:2009-03-06 Revised:2009-04-22 Online:2009-12-15 Published:2012-03-27
  • Contact: GU Ning-Yan E-mail:ningyangu@139.com

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摘要：

目的 探讨IFN-γ受体2（IFN-γR2）基因氨基酸位点Gln64Arg多态性与中国汉族寻常性银屑病的相关性。方法 采用PCR-限制性片段长度多态性（RFLP）方法及DNA测序方法，检测苏皖地区182例汉族寻常性银屑病患者和114例正常人对照IFN-γR2基因Gln64Arg位点多态性。结果 寻常性银屑病患者与正常人对照组IFN-γR2基因Gln64Arg多态性位点各基因型及等位基因频率分布差异无统计学意义（P > 0.05）。伴甲损害银屑病组与无甲损害银屑病组之间Gln64/Gln64基因型分布频率分别为57.5%和38.1%，差异有统计学意义（χ2 = 5.33，P = 0.02）；Arg64等位基因（Gln64等位基因）分布频率分别为19.3%（80.7%）和30%（70%），差异也有统计学意义（χ2 = 5.03，P = 0.02）。伴甲损害的银屑病患者组Gln64/Arg64基因型分布频率为29.8%，正常人对照组为49.1%，两组间分布差异有统计学意义（χ2 = 5.48，P = 0.01）；Gln64/Gln64基因型分布频率在两组间分别为57.5%和35.1%，差异有统计学意义（χ2 = 6.23，P = 0.01）；无甲损害组与正常人对照组之间差异则无统计学意义。在以上呼吸道感染为诱因或加重因素的银屑病组与非上呼吸道感染银屑病组之间Arg64/Arg64基因型分布频率分别为33.3%和15.5%，差异有统计学意义（χ2 = 4.94，P = 0.03）；Arg64等位基因（Gln64等位基因）分布频率为51.9%（48.1%）和35.2%（64.8%），差异也有统计学意义（χ2 = 5.46，P = 0.02）。结论 IFN-γR2基因Gln64Arg多态性位点可能与寻常性银屑病患者是否伴有甲损害有关，也可能与以上呼吸道感染为诱因或加重因素的寻常性银屑病有关。

关键词: 受体，干扰素

Abstract:

Objective To assess the association between the amino acid polymorphism （Arg64Gln） within the interferon-γ receptor 2 gene（IFN-γR2） and psoriasis vulgaris in Chinese Hans. Methods Blood samples were collected from 182 patients with psoriasis vulgaris and 114 healthy human controls in Jiangsu and Anhui provinces. The amino acid polymorphism（Arg64Gln） within the IFN-γR2 was examined by PCR-restriction fragment length polymorphism （RFLP） and DNA sequencing. Results No significant difference was observed in the amino acid polymorphism （Arg64Gln） within the IFN-γR2 between the psoriatic patients and healthy controls （P > 0.05）. There was a significant difference between patients with nail involvement and those without in the frequency of Gln64/Gln64 genotype （57.5% vs 38.1%, χ2 = 5.33, P < 0.05）, and Arg 64 （Gln64） allele ［19.3% （80.7%） vs 30% （70%）, χ2 = 5.03, P < 0.05］. The frequencies of Gln64/Arg64 genotype and Gln64/Gln64 genotype in psoriatic patients with nail involvement significantly differed from those in the controls （29.8% vs 49.1%, χ2 = 5.48, P < 0.05; 57.5% vs 35.1%, χ2 = 6.23, P < 0.05）, while no significant difference was found between the psoriatic patients without nail involvement and controls. Moreover, significant difference was noted between patients with prior upper respiratory tract infection （as inducements） and those without in the frequency of Arg64/Arg64 genotype （33.3% vs 15.5%, χ2 = 4.94, P < 0.05） and Gln64 （Arg64） allele ［51.9% （48.1%） vs 35.2% （64.8%）, χ2 = 5.46, P < 0.05］. Conclusion The amino acid polymorphism （Arg64Gln） within the IFN-γR2 may be associated with the nail involvement and upper respiratory tract infection in patients with psoriasis vulgaris.