中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (1): 57-58.

• 短篇论著 • 上一篇    下一篇

过敏原免疫治疗对慢性荨麻疹患者单一核细胞IL-2、4受体表达的影响

宋彪 魏丽虹 郑利雄 熊峰 房思宁 杜晓红 郭棱棱 梅忠喜 童秋生   

  1. 深圳市人民医院皮肤科 深圳市人民医院皮肤科 深圳市人民医院皮肤科 深圳市人民医院皮肤科 深圳市人民医院皮肤科 深圳卫生学校门诊部皮肤科 深圳市人民医院皮肤科
  • 收稿日期:2008-04-18 修回日期:2008-05-21 出版日期:2009-01-15 发布日期:2009-01-15
  • 通讯作者: 宋彪 E-mail:biaosonggx@sohu.com

Effect of allergen immunotherapy on the expression of interleukin-2 and -4 receptors by mononuclear cytes in patients with chronic urticaria

SONG Biao Li-hong WEI Ling-ling GUO   

  • Received:2008-04-18 Revised:2008-05-21 Online:2009-01-15 Published:2009-01-15
  • Contact: SONG Biao E-mail:biaosonggx@sohu.com

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摘要：

目的 通过对比过敏原免疫治疗（AIT）前后慢性特发性荨麻疹（CIU）患者体内IL-2、4受体的变化，初步探索AIT对CIU患者免疫的影响，CIU的发病机制及AIT治疗CIU的机制。方法 临床选择35例CIU患者，实验组20例，对照组15例。实验组给予AIT干预。对照组仅抗组胺药对症治疗。实验前后分别采集静脉全血，提取总的RNA，逆转录-PCR扩增IL-2R、4R及内参照β肌动蛋白mRNA的片断。目的条带的峰面积除以内参照β肌动蛋白的条带峰面积，作为目的蛋白mRNA表达的半定量值，结果用 表示，统计分析采用配对资料t检验。结果 实验组实验前后IL-2R mRNA的表达量分别为0.356±0.111，0.370±0.092；IL-4R mRNA的表达量分别为0.483±0.087，0.474±0.076。对照组实验前后IL-2R mRNA的表达量分别为0.330±0.111，0.335±0.093；IL-4R mRNA的表达量分别为0.511±0.080，0.527±0.086。通过配对t检验分析，比较实验前后每组IL-2R、4R mRNA的表达量，P值均大于0.05，没有统计学意义。实验组中，AIT疗效达到良好及其以上的共13例，以13例作为一组分析，实验前后其IL-2R mRNA表达量分别为0.338±0.122，0.359±0.104；IL-4R mRNA表达量分别为0.485±0.092，0.471±0.084。通过配对t检验分析，P值均大于0.05，没有统计学意义。结论 AIT疗效良好的实验组在实验前后IL-2R，IL-4R mRNA的表达没有统计学差异，说明CIU的发病并不是单纯的Th2表达模式，同时说明免疫偏移不是AIT治疗CIU的主要机制。

Abstract:

Objective 　To compare the mRNA expression of interleukin 2 (IL-2) and 4 (IL-4) receptors in the patients with chronic idiopathic urticaria (CIU) before and after allergen immunotheropy (AIT).　Methods 35 patients with CIU were enrolled in this study, divded into group 1 with AIT and group 2 without AIT. The peripheral blood was extracted before and after test. Reverse transcription-polymerase chain reaction (RT-PCR) technique was applied to semi-quantitatively analyze the mRNA expression of IL-2 and IL-4 receptors. Results Before and after test, the mRNA expressions of IL-2 receptor were 0.356±0.111，0.370±0.092 respectively in group 1, and 0.330±0.111，0.335±0.093 in group 2. The mRNA expression of IL-4 receptor were 0.483±0.087，0.474±0.076 in group 1, and 0.511±0.080，0.527±0.086 in group 2. It showed no significant difference before and after test for IL-2 or IL-4 receptor in each group. Among the 13 cases of group 1 with good responses to AIT, the expressions of IL-2 receptor were 0.338±0.122，0.359±0.104 before and after test. And the expressions of IL-4 receptor were 0.485±0.092，0.471±0.084. There was also no significant difference. Conclusion Th2 model is not the mechanism of CIU. Re-balance between Th1 and Th2 is not the main mechanism of AIT to treat CIU.