中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (8): 554-555.

• 技术与方法 • 上一篇    下一篇

变性高效液相色谱法快速检测常见念珠菌

莫友 王建琴 王汉平 王劭晟 方锐华 谢健晋 赵子文 黄侃   

  1. 广州医学院附属广州市第一人民医院 广州市第一人民医院皮肤科 广州市第一人民医院血液内科 广州市第一人民医院皮肤科
  • 收稿日期:2008-01-09 修回日期:2008-02-27 发布日期:2008-08-15
  • 通讯作者: 莫友 E-mail:mouyuo@163.com

Detection of common Candida spp. by denaturing high-performance liquid chromatography

  

  • Received:2008-01-09 Revised:2008-02-27 Published:2008-08-15

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摘要： 目的 应用变性高效液相色谱法（DHPLC）快速检测和鉴定7种常见的念珠菌。方法 对7种常见致病性念珠菌标准株的rDNA 内转录间隔区进行PCR扩增，对扩增产物分单种和混合两种方式分别进行DHPLC分析。结果 两种方式鉴定的7种念珠菌均能产生特异性峰形图谱；整个鉴定过程约7小时。结论 PCR-DHPLC方法检测和鉴定常见的致病性念珠菌具有快速、简便、特异和高通量的特点，并且能同时检测混合标本中不同的致病菌株，可为临床诊断深部念珠菌感染提供一种可靠的方法。

关键词: 念珠菌属, 变性高效液相色谱, 聚合酶链反应

Abstract: Objective Use of Denaturing High-Performance Liquid chromatography for Rapid Detection and Identification of seven Common Pathogenic Candida Species. Methods PCR was used to amplify the internal transcribed spacer region of ribosomal DNA from 7 type strains of Pathogenic Candida Species，and the single and mixed products were analyzed respectively. Results Both single and mixed products show unique peak profiles，The whole process tool about 7 h to complete the identification. Conclusion PCR-DHPLC is fast, simple, specific and high-through for rapid detection and identification of common pathogenic Candida species. Moreover, it can detect different species of mixed samples, which may provide a reliable approach to clinical diagnosis of deep Candida infection.

Key words: Candida, Denaturing High-Performance Liquid chromatography, polymerase chain reaction