中华皮肤科杂志

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PCR-RFLP分析鉴定皮肤组织中的分枝杆菌

蔡林, 赵亭, 张建中   

  1. 北京大学人民医院皮肤科, 100044
  • 收稿日期:2006-07-03 出版日期:2007-06-15 发布日期:2007-06-15
  • 基金资助:
    北京大学985基金

Identification of mycobacterium in skin lesions by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)

CAI Lin, ZHAO Ting, ZHANG Jian-zhong   

  1. Department of Der-matology, Peking University People's Hospital, Beijing 100044, China
  • Received:2006-07-03 Online:2007-06-15 Published:2007-06-15

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摘要： 目的 探讨用PCR限制性片段长度多态性(PCR-RFLP)分析的方法快速检测皮肤感染性肉芽肿组织中分枝杆菌。方法 皮肤感染性肉芽肿患者组织标本9份.提取组织DNA,进行PCR扩增,PCR产物分别用BstEⅡ和HaeⅢ2种限制性内切酶进行酶切,3%Nusieve 3:1琼脂糖凝胶电泳,进行限制性片段长度多态性分析,并与培养结果进行比较。结果 从5份临床诊断为游泳池肉芽肿的组织标本中检测出海分枝杆菌,从另4份诊断为皮肤感染性肉芽肿的标本中检测出2份结核分枝杆菌和2份脓肿分枝杆菌,此9份结果与培养鉴定结果完全一致。结论 用PCR-RFLP方法可以快速鉴定皮肤感染性肉芽肿组织中的分枝杆菌。

关键词: 分枝杆菌感染, 分枝杆菌属, 多态性, 限制性片段长度

Abstract: Objective To establish a rapid approach for detection and identification of mycobac-terium from cutaneous infectious granuloma.Methods Tissue specimens were obtained from granulomatous lesions of 9 patients with suspected mycobacterial infection.DNA was extracted and PCR was performed to amplify the 65 kD heat shock protein(hsp65)gene of mycobacterium.PCR products were assessed by restriction fragment length polymorphism(RFLP)with endonucleases BsteⅡand HaeⅢ.Results M.marinum was detected from 5 cases of swimming pool granuloma,M.tuberculosis in 2 cases,and M.abscessus in 2 cases of cutaneous infectious granuloma.All the results were confirmed by mycobacterial culture.Conclusion PCR-RFLP is a rapid method for the identification of mycobacterium in cutaneous infectious granuloma.

Key words: restriction fragment length, Mycobacterium infections, Mycobacterium, Polymorphism