中华皮肤科杂志

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皮肤鳞状细胞癌上皮型钙黏着蛋白基因甲基化与蛋白表达的关系

魏志平, 李艳, 刘彦群   

  1. 江苏省徐州医学院附属医院皮肤科, 221002
  • 收稿日期:2006-08-24 出版日期:2007-06-15 发布日期:2007-06-15

Relationship between the promoter methylation status and protein expression of E-cadherin in cutaneous squamous cell carcinoma

WEI Zhi-ping, LI Yan, LIU Yan-qun   

  1. Department of Dermatology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China
  • Received:2006-08-24 Online:2007-06-15 Published:2007-06-15

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摘要： 目的 探讨皮肤鳞状细胞癌(SCC)组织中上皮型钙黏着蛋白(E-cadherin)基因启动子甲基与蛋白表达的关系。方法 运用甲基化特异性PCR(MSP)方法检测10例正常皮肤组织和43例皮肤SCC中E-cadherin基因启动子区域甲基化状况,并用免疫组化S-P法检测E-cadherin蛋白表达情况。结果 在43例皮肤SCC中有30例(69.7%)E-cadherin表达减弱或缺失,31例无淋巴结转移的皮肤SCC组织中19例(61.3%)表达减弱或缺失,12例有淋巴结转移的皮肤SCC组织中11例表达减弱或缺失(91.7%)。43例皮肤SCC中有17例(39.5%)出现E-cadherin基因启动子甲基化,有淋巴结转移组中甲基化率明显高于无淋巴结转移组;皮肤SCC组织中有E-cadherin基因启动子甲基化者,E-cadherin表达明显低于无甲基化者。结论 E-cadherin的表达异常可能与皮肤SCC的转移有关,E-cadherin基因启动子甲基化可能是导致E-cadherin表达下调的主要原因。

关键词: 癌, 鳞状细胞, 钙黏着糖蛋白类, 甲基化

Abstract: Objective To detect the promoter methylation status of E-cadherin gene in cutaneous squamous cell carcinoma and to evaluate its possible relation to the protein expression of E-cadherin.Methods Tissue specimens were resected from 10 normal human controls and 43 patients with SCC,and examined for promoter methylation status of E-cadherin gene by methylation-specific PCR(MSP).The protein expression of E-cadherin was measured by immunohistochemistry SP method.Results The E-cadherin expression was decreased or absent in 69.7%(30/43)of all SCC cases,61.3%(19/31)of SCC cases without lymphonode-metastasis and 91.7%(11/12)of SCC cases with lymphnode-metastasis.The promoter methylation of E-cadherin was detected in 39.5%(17/43)of all SCC specimens.Patients with SCC with lymph node metastases showed a higher frequency of E-cadherin promoter methylation than those without.The protein expression of E-cadherin was lower in patients with E-cadherin promoter methylation than those without.Conclusion The abnormal expression of E-cadherin,likely to be induced by its promoter methylation,may be related to the metastasis of SCC.

Key words: Carcinoma, squamous cell, Methylation, Cadherins