中华皮肤科杂志 ›› 2007, Vol. 40 ›› Issue (3): 158-160.

• 论著 • 上一篇    下一篇

毛囊黏蛋白沉积症与蕈样肉芽肿T细胞受体基因重排的对比研究

李远1, 周平1, 刘跃华2, 昌红3, 李宝玉3   

  1. 1. 北京世纪坛医院皮肤科, 100038;
    2. 北京协和医院皮肤科;
    3. 北京世纪坛医院病理科, 100038
  • 收稿日期:2006-03-22 出版日期:2007-03-15 发布日期:2007-03-15

A comparison study of T lymphocytes receptor gene rearrangement between follicular mueinosis and mycosis fungoides

LI Yuan1, ZHOU Ping1, LIU Yue-hua2, CHANG Hong3, LI Bao-yu3   

  1. Department of Dermatology, Beijing Shijitan Hospital, 100038, China
  • Received:2006-03-22 Online:2007-03-15 Published:2007-03-15

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摘要： 目的 研究毛囊黏蛋白沉积症与蕈样肉芽肿的关系.方法 从8例特发性毛囊黏蛋白沉积症、3例毛囊黏蛋白沉积症合并蕈样肉芽肿和1例蕈样肉芽肿患者获得病变组织,将其埋于石蜡中.设计T淋巴细胞受体(TCR)可变区引物Vγ1-8/A,Vγ10,Vγ11及β,利用PCR方法分析了12例患者组织中T淋巴细胞受体基因重排的情况.结果 8例特发性毛囊黏蛋白沉积症中1例,3例毛囊黏蛋白沉积症合并蕈样肉芽肿和1例蕈样肉芽肿患者皮损TCR Vγ1-8/A呈单克隆性.结论 对于年龄较大、病程较长的毛囊黏蛋白沉积症患者,应行TCRγ基因重排检查,以排除淋巴瘤.

关键词: N-乙酰氨基葡萄糖, 成纤维细胞, 透明质酸, 透明质酸合成酶, 真菌病,覃样, 毛囊豁蛋白病, 基因重排,γ链T细胞抗原受体

Abstract: Objective To explore the relationship between follicular mucinosis and mycosis fungoides(MF).Methods Tissuc specimens were obtained from 8 cases of idiopathic follicular mucinosis, 3 cases of follicular mucinosis accompanied with MF and 1 case of MF, then embedded in paraffin.The primers, including Vγl-8/A,Vγ10, Vγ11 and a were designed to target the variable domain of T lymphocyte receptor(TCR).The specimens were subjected to PCR with the above primers for the analysis of TCR gcnc rearrangement.Results A monoclonal rearrangement of TCRγI-8/A was found in 1 case of idiopathic follicular mucinosis, 3 cases of follicular mucinosis accompanied by MF, and 1 case of MF.Conclusion Detection of TCRγ gene rearrangement is recommended for aged patients with long-course of follicular mucinosis to rule out potential lymphoma.

Key words: Mycosis fungoides, fungoides, Follicular mucinosis, Gene rearrangement, gamma-chain T-cell antigen receptor