中华皮肤科杂志 ›› 2007, Vol. 40 ›› Issue (3): 152-154.

• 论著 • 上一篇    下一篇

皮肤基底细胞癌T钙黏蛋白的表达及其DNA异常甲基化分析

于波1, 程滨珠1, 钟绮丽1, 邵勇1, 廖康煌2   

  1. 1. 北京大学深圳医院皮肤性病科, 深圳, 518036;
    2. 复旦大学附属华山医院皮肤科
  • 收稿日期:2006-03-24 出版日期:2007-03-15 发布日期:2007-03-15
  • 基金资助:
    广东省卫生厅资助课题(A2004627)

The expression and aberrant methylation of T-cadherin in cutaneous basal cell carcinoma

YU Bo1, CHENG Bin-zhu1, ZHONG Qi-li1, SHAO Yong1, LIAO Kang-huang2   

  1. Department of Dermatology and Venereology, Peking University Shenzhen Hospital, Shenzhen 518036, Guangzhou, China
  • Received:2006-03-24 Online:2007-03-15 Published:2007-03-15

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摘要： 目的 检测皮肤基底细胞癌(BCC)中T钙黏蛋白的表达以及异常甲基化.方法 采用免疫组化法和CpG岛甲基化特异性PCR方法,对29例BCC和19例正常人暴光部位皮肤的组织标本进行T钙黏蛋白的表达及甲基化检测.结果 正常皮肤皆表达T钙黏蛋白,29例BCC中8例(27.59%)检测到T钙黏蛋白表达(P<0.05),9例BCC(31.03%)和19例正常皮肤中的1例(5.26%)检测到T钙黏蛋白启动子区域的异常甲基化(P<0.05).结论 T钙黏蛋白表达降低与皮肤基底细胞癌的发病机制相关,T钙黏蛋白启动子区域的异常甲基化与蛋白低表达可能相关.

关键词: 尖锐湿疣, 外周血单核细胞, Toll样受体, 流式细胞仪, 癌,鳞状细胞, 甲基化, 钙粘着糖蛋白类

Abstract: Objective To detect the expression and aberrant mcthylation of T-cadherin in cutaneous basal cell carcinoma(BCC).Methods Skin tissues were resected during operation in light-exposed sites from 29 patients with BCC and 19 normal controls.The expression and aberrant mcthylation of T-cadherin were tested by immunohistochemical staining and methylation-specific polymerase chain reaction (MSP),respectively.Results Immunohistochemical staining showed that T-cadhcrin was expressed in 8 of 29(27.59%)BCC specimens, whereas in all(100%)normal controls, and there was a significant difference in T-cadherin positivity rates between the two groups(X2=24.46, P<0.05).The aberrant methylation of T-cadherin promoter region was detected in 9 BCC(37.93%)and in 1(5.26%)control specimen with significant difference between them(X2=4.62, P<0.05).Conclusion The decrease of T-cadherin expression, likely to be relavent to the aberrant methylation of T-cadherin promoter, might contribute to the pathogenesis of BCC.

Key words: Carcinoma, basal cell, Mcthylation, Cadherins