中华皮肤科杂志

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强脉冲光照射对皮肤成纤维细胞活性及其分泌基质金属蛋白酶的影响

许阳, 吴迪, 骆丹, 陈斌, 薛筑云   

  1. 南京医科大学第一附属医院皮肤性病科 210029
  • 收稿日期:2007-02-06 出版日期:2007-12-15 发布日期:2007-12-15
  • 通讯作者: 骆丹,email:daniluo2005@yahoo.com.cn E-mail:daniluo2005@yahoo.com.cn
  • 基金资助:
    国家自然科学基金(30371294)

Effects of intense pulsed light on cell viability of and secretion of matrix metalloproteinases by cultured human fibroblasts

XU Yang, WU Di, LUO Dan, CHEN Bin, XUE Zhu-yun   

  1. Department of Dermatology and Venereology, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029
  • Received:2007-02-06 Online:2007-12-15 Published:2007-12-15

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摘要： 目的 探讨强脉冲光(IPL)照射对培养状态下的人成纤维细胞形态、增殖活性以及分泌基质金属蛋白酶l(MMP-1)和基质金属蛋白酶2(MMP-2)的影响.方法 由健康人皮肤标本中分离培养成纤维细胞,用一定剂量IPL分别照射后进行培养,光学显微镜观察成纤维细胞形态学变化,MTY法检测细胞增殖活性,ELISA法检测MMP-1及MMP-2水平.结果 成纤维细胞经IPL照射后形态无明显改变;能量密度为18,29,35J/cm2的IPL均可明显提高成纤维细胞活性,但无剂量依赖性.IPL照射后成纤维细胞MMP-1及MMP-2分泌水平无明显改变.结论 IPL照射能增强成纤维细胞增殖活性.

关键词: 强脉冲光, 成纤维细胞, 基质金属蛋白酶类

Abstract: Objective To investigate the effects of intense pulsed light(IPL)on the cell viability of and secretion of MMP-1 and MMP-2 by cultured human fibroblasts.Methods Fibroblasts were isolated from healthy infant foreskins,and irradiated with 18,29,35 J/cm2 of IPL respectively.The morphological changes of cells were observed by light microscopy.MTT assay was used to detect the proliferation and viability of the fibroblasts.The secretion levels of MMP-1 and MMP-2 were determined by ELISA.Results IPL irradiation showed no noticeable effects on the morphology of fibroblasts.Proliferation of fibroblasts was increased at 24 h and 48 h after IPL irradiation of 18,29,35 J/cm2;however,no correlation with the irradiation dose was observed.IPL irradiation showed no effect on MMP-1 and MMP-2 secretion by the fibroblasts. Conclusion IPL irradiation could induce the proliferation of cultured human fibroblasts.

Key words: Intense pulse light, Fibroblasts, Matrix metalloproteases