中华皮肤科杂志

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抗角蛋白16抗体对角质形成细胞外皮蛋白、Toll样受体2和4的调节

武彩霞, 郑志忠, 阎春林   

  1. 复旦大学附属华山医院皮肤科, 上海, 200040
  • 收稿日期:2006-11-07 出版日期:2007-10-15 发布日期:2007-10-15

Regulation of involucrin,Toll like receptors-2 and-4 expressions in keratinocytes by anti-keratin 16 antibody

WU Cai-xia, ZHENG Zhi-zhong, YAN Chun-lin   

  1. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2006-11-07 Online:2007-10-15 Published:2007-10-15

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摘要： 目的 研究抗角蛋白16抗体对角质形成细胞分化、Toll样受体2和4的调节。方法 将抗角蛋白16抗体加入培养的角质形成细胞中,以同种型抗体作对照,研究外皮蛋白和Toll样受体2和4mRNA表达。结果 抗角蛋白16抗体作用角质形成细胞6h、24h、36h后,Toll样受体2mRNA表达分别上调1.73、1.60、2.52倍:12 h和36h后Toll样受体4mRNA表达分别上调3.62和2.21倍;12 h、36h后外皮蛋白mRNA表达分别上调2.33倍和2.0倍。结论 抗角蛋白16抗体可能是联系Toll样受体2和4与角质形成细胞分化的重要环节。

关键词: 角蛋白细胞, Toll样受体2, Toll样受体4, 抗角蛋白16抗体, 外皮蛋白

Abstract: Objective To study the regulation of involucrin,Toll like receptors(TLR)-2 and-4 expressions in keratinocytes by anti-keratin 16 antibody.Methods Keratinocytes were isolated from the foreskins of children,and subjected to primary culture.The cultured keratinocytes were incubated with antikeratin 16 antibody,or with isotype antibody as control,for various durations.Real-time PCR was used to detect the mRNA expression of TLR2,TLR4 and involucrin in keratinocytes.Results After the co-culture of keratinocytes with anti-keratin 16 antibody,TLR2 mRNA expression was increased by 1.73,1.60 and 2.52 folds at 6 h,24 h and 36 h respectively,TLR4 mRNA expression by 3.62,2.21 folds at 12 h and 36 h respectively,and involucrin mRNA expression by 2.33,2.0 folds at 12 h and 36 h respectively.Conclusion Anti-keratin 16 antibody may be a pivotal link for the expression of TLR2 and TLR4 in the differentiation of keratinocytes.

Key words: Keratinocytes, Toll-like receptor 2, Toll-like receptor 4, anti-keratin 16 antibodies, Involucrin