抗Dsg—3 EC3—4的单链抗体片段在寻常型天疱疮鼠模型中的作用

中华皮肤科杂志

抗Dsg—3 EC3—4的单链抗体片段在寻常型天疱疮鼠模型中的作用

赵肖庆, 薛峰, 潘萌, 李卫平, 郑捷

上海交通大学医学院瑞金医院皮肤科 200025

收稿日期:2005-09-15 出版日期:2006-04-15 发布日期:2006-04-15
基金资助:
国家自然科学基金(30170858);上海市卫生局“百人计划”(98B37)资助

The effect of single chain variable fragment antibody to EC3-4 fragment of desmoglein 3 in a mouse model of pemphigus vulgaris

ZHAO Xiao-qing, XUE Feng, PAN Meng, LI Wei-ping, ZHENG jie

Department of Dermatology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China

Received:2005-09-15 Online:2006-04-15 Published:2006-04-15

摘要/Abstract

摘要： 目的探讨抗桥粒芯糖蛋白3(Dsg-3)EC3-4的单链抗体片段(ScFv)在寻常型天疱疮(PV)鼠模型中的作用。方法在不同时间点。将抗Dsg-3 EC3-4的ScFv通过皮下注射至BALB/c新生鼠,从临床表现、组织病理和免疫荧光三方面评价其对PV鼠模型构建的影响。结果单独注射抗Dsg-3 EC3-4的ScFv不能在BALB/c新生鼠中诱导出PV的改变,任何时间点注射抗Dsg-3 EC3-4的ScFv都不能阻断PV患者血清对BALB/c新生鼠的致病作用。结论单独注射抗Dsg-3 EC3-4的ScFv缺乏致病性,也不能阻断PV患者血清对BALB/c新生鼠的致病作用。

关键词: 天疱疮, 寻常, 抗体, 抗桥粒芯糖蛋白, 模型, 动物

Abstract: Objective To study the effect of single chain variable fragment (ScFv) antibody to EC3-4 fragment of desmoglein (Dsg) 3 in a mouse model of pemphigus vulgaris.Methods The ScFv antibody to EC3-4 fragment of Dsg-3 was injected subcutaneously into neonatal BALB/c mice at different time points;the mice were then evaluated clinically,histopathologically and by direct immunoflorescence examination for the development of lesions.Results When injected alone,the ScFv antibody did not induce the appearance of key clinical features of pemphigus vulgaris.The antibody also did not prevent the development of pemphigus vulgaris features induced by sera of patients with pemphigus vulgaris,regardless of the time point of injection of ScFv antibody.Conclusion The ScFv antibody to EC3-4 fragment of Dsg-3 lacks pathogenicity in neonatal BALB/c mice,and also could not inhibit the development of lesions induced by sera from patients with pemphigus vulgaris.

Key words: Pemphigus vulgaris, Antibodies,anti-Dsg3, Models,animal

赵肖庆, 薛峰, 潘萌, 李卫平, 郑捷. 抗Dsg—3 EC3—4的单链抗体片段在寻常型天疱疮鼠模型中的作用[J]. 中华皮肤科杂志, 2006, 39(6): 338-340.

ZHAO Xiao-qing, XUE Feng, PAN Meng, LI Wei-ping, ZHENG jie. The effect of single chain variable fragment antibody to EC3-4 fragment of desmoglein 3 in a mouse model of pemphigus vulgaris[J]. Chinese Journal of Dermatology, 2006, 39(6): 338-340.

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