中华皮肤科杂志

• 论著 • 上一篇    下一篇

核因子κB和c-myc在尖锐湿疣皮损角质形成细胞中的表达

苏明1, 程浩2, 陈洵礼1, 吴黎明1, 沈斌1   

  1. 1. 杭州市第一人民医院皮肤科 310006;
    2. 浙江大学医学院附属邵逸夫医院皮肤科
  • 收稿日期:2005-06-03 出版日期:2006-04-15 发布日期:2006-04-15

Expression of NF-κB and c-myc in keratinocytes of condyloma acuminata lesions

SU Ming1, CHENG Hao2, CHEN XUN-li1, WU LI-ming1, SHEN Bin1   

  1. Department of Dermatology, First Hospital of Hangzhou, Hangzhou 310006, China
  • Received:2005-06-03 Online:2006-04-15 Published:2006-04-15

PDF (PC)

83

赞

摘要： 目的 探讨核因子-κB(NF-κB)和c-myc在尖锐湿疣皮损角质形成细胞中的表达。方法 采用原位杂交方法检测尖锐湿疣50例,确定为HPV6/11阳性;采用免疫组化En Vision二步法检测50例尖锐湿疣患者皮损和25例正常人皮肤(包皮)中NF-κB和c-myc的表达及分布。结果 ①与正常人皮肤对照组相比,尖锐湿疣皮损角质形成细胞中NF-κB和c-myc的表达均有不同程度增强(前者U=6.286,P<0.01;后者U=4.356,P<0.01)。NF-κB表达主要分布于棘细胞层,阳性信号定位于胞浆。c-myc表达主要分布于表皮全层或棘细胞层和基底层,阳性信号定位于胞核。②尖锐湿疣皮损中,NF-κB与c-myc的表达呈正相关(r=0.89,P<0.01)。结论 在HPV6/11感染时,角质形成细胞中转录因子NF-κB和c-myc的表达增高,可能均参与HPV感染细胞增殖的调控。

关键词: 尖锐湿疣, 乳头状瘤病毒, 人, NF-κB, 原癌基因蛋白质c-myc

Abstract: Objective To investigate the expression of NF-k B and c-myc in keratinocytes of HPV infected condyloma acuminata (CA) lesions.Methods Fifty cases of HPV 6/11 positive CA were diagnosed by in situ hybridization.The expression and distribution of NF-k B and c-myc were detected by immunohistochemistry technique (EnVision) with CA lesions and 25 normal human skins (foreskins).Results ②The expression of NF-k B and c-myc was significantly higher in keratinocytes of CA lesions than those of normal controls (P<0.01).NF-k B was mainly expressed in spinous layers of CA lesions, and most positive signals located significantly in the cytoplasm.C-myc was mainly expressed in the whole epidermis or in spinous and basal layers in CA lesions,and positive signals located in the nuclei.②There was a positive correlation between the expression of NF-k B and c-myc in CA lesions (r=0.89,P<0.01).Conclusions The study demonstrates that the expression of both NF-k B and c-myc is significantly higher in HPV 6/11 positive CA than in normal skin.The data suggest that transcriptional factor NF-k B and c-myc may play a role in the regulation of proliferation of HPV 6/11 infected cells.

Key words: Condyloma acuminata, Papillomavirus,human, NF-kappa B, Proto-oncogene proteins c-myc