中华皮肤科杂志

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人乳头瘤病毒11型DNA疫苗协同CD80基因诱导小鼠特异性免疫反应

李莉华1,2, 黄朝晖1,2, 郭子健1,2, 刘志辉1,2, 任金冬1,2, 宋明旭1,2, 周希科1,2, 王飞3, 毕志刚3   

  1. 1. 苏州大学附属无锡第四医院中心实验室 无锡 214062;
    2. 无锡市肿瘤防治研究所;
    3. 南京医科大学附属第一医院皮肤科
  • 收稿日期:2006-02-13 出版日期:2006-04-15 发布日期:2006-04-15
  • 基金资助:
    江苏省重点学科基金(135-03);江苏省重点学科开放招标项目(WK200221)

Enhancement of human papillomavirus type 11 DNA vaccine-induced specific immune response by co-immunization with CD80 co-stimulatory gene

LI Li-hua1,2, HUANG Zhao-hui1,2, GUO Zhi-jian1,2, LIU Zhi-hui1,2, REN Jin-dong1,2, SONG Ming-xu1,2, ZHOU Xi-ke1,2, WANG Fei3, BI Zhi-gang3   

  1. Wuxi No.4 People's Hospital Affiliated to Suzhou University, Wuxi Cancer Institute, Wuxi 214062, China
  • Received:2006-02-13 Online:2006-04-15 Published:2006-04-15

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摘要： 目的 探讨人乳头瘤病毒(HPV)11E7、L1-E7DNA疫苗质粒协同共刺激分子CD80诱导的特异性免疫反应.方法 将所构建的pcDNA3.1(+)/HPV11E7、E7-CD80、L1-E7DNA疫苗质粒分别经肌内注射免疫小鼠,并设L1-E7质粒与CD80质粒共同注射组,PCR和RT-PCR检测质粒DNA的组织分布和目的基因RNA转录,MTT法测脾淋巴细胞增殖活性,ELISA检测脾淋巴细胞培养上清细胞因子的表达水平及血清抗体的水平.结果 免疫小鼠后,质粒主要分布在注射部位.DNA疫苗诱导机体产生特异性脾淋巴细胞增殖和分泌IL-2、IFN-γ增加,产生HPV11E7 IgG/L1 IgG抗体;与CD80基因嵌合后,脾淋巴细胞增殖和分泌活性显著增强;与CD80联合免疫组所诱导的细胞免疫反应和体液免疫均显著强于L1-E7单独免疫组.结论 HPV11DNA疫苗能诱导小鼠产生特异性免疫反应,CD80可加强其免疫效果.

关键词: 乳头状瘤病毒,人, 抗原,CD80, 疫苗,DNA

Abstract: Objective To investigate the specific immune response induced by DNA vaccinal plasmid of human papillomavirus type 11 (HPV11)E7,L1-E7 and enhancement of immune reaction by CD80 co-stimulatory gene.Methods The constructed pcDNA3.1 (+)/HPV11E7,E7-CD80 and L1-E7 DNA vaccinal plasmid,or combination of CD80 and L1-E7 plasmids,were injected intramuscularly into mice,respectively.The distribution of plasmid DNA and transcription of RNA in tissue were analyzed by PCR and RT-PCR,respectively.The lymphocyte proliferation activity was detected by MTT assay.The level of cytokines was determined by ELISA in supernatants of cultured spleen cells and antibodies in serum.Results After immunization,the recombinant plasmid was mainly located in the injection site.Specific anti-HPV 11 E7 IgG/L1 IgG antibodies,lymphocyte proliferation and secretions of cytokines IL-2 and IFN-γwere induced by injection of DNA vaccine.The levels of lymphocyte proliferation and cytokine secretion were significantly higher in groups of HPV11E7 in recombinant with CD80 co-stimulatory gene than those without CD80.The levels of specific cellular and humoral immunity were also significantly increased in group with the combination of L1-E7 plasmid and CD 80 than those without CD80.Conclusions HPV11DNA vaccination could induce specific cellular and humoral immunity.CD80 co-stimulatory gene may represent an attractive adjuvant for enhancement of the specific immune responses.

Key words: Papillomavirus,human, Antigens,CD80, Vaccines,DNA