中华皮肤科杂志

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衣原体GPIC噬菌体衣壳蛋白Vp1的克隆、表达和鉴定

刘全忠, 姚卫锋, 齐蔓莉, 李燕, 王惠平, 田敬群   

  1. 天津医科大学总医院皮肤性病科 300052
  • 收稿日期:2006-02-27 出版日期:2006-04-15 发布日期:2006-04-15
  • 基金资助:
    国家自然科学基金资助项目(30671879);天津市自然科学基金资助项目(023612111)

Cloning,expression and identification of chlamydial GPIC capsid Vp1 protein

LIU Quan-zhong, YAO Wei-feng, QI Man-li, LI Yan, WANG Hui-ping, TIAN Jin-qun   

  1. Department of Dermatology, General Hospital of Tianjin Medical University, Tianjin 300052, China
  • Received:2006-02-27 Online:2006-04-15 Published:2006-04-15

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摘要： 目的 获得衣原体GPIC噬菌体衣壳蛋白Vp1基因及其蛋白.方法 提取噬菌体ΦCPG1及其核酸,并对Vp1克隆、表达,鉴定.结果 所获得的Vp1基因片段经测序,长度为1661bp,检索确认其序列与Genebank一致.对重组质粒进行诱导表达,SDS-PAGE和蛋白质印迹均显示获得相对分子质量约62000的衣原体GPIC噬菌体衣壳蛋白Vp1.结论 成功获得了噬菌体衣壳蛋白Vp1,为进一步研究打下基础.

关键词: 衣原体噬菌体, Vp1蛋白, 重组, 表达

Abstract: Objective To clone,express,and identify chlamydial GPIC capsid Vp1 gene and protein.Methods The complete sequence of Vp1 gene fromΦCPG1 phage was amplified.The amplicor was cut by restriction endonuclease,linked to plasmid vector,and transformed into E.coli.The expressed protein of recombinant Vp1 was purified and identified.Results The recombinant 1 661 bp gene was sequenced and proved to beΦCPG1 Vp1 by searching Genebank.A 62 kDa capsid protein was expressed and confirmed by SDS-PAGE and Western blot.Conclusion The recombinant Vp1 seems to be a highly conserved and specific marker for chlamydial phage.

Key words: Chlamydiaphage, Vp1 protein, expression