中华皮肤科杂志 ›› 2006, Vol. 39 ›› Issue (11): 639-641.

• 论著 • 上一篇    下一篇

丹皮酚在体外对人黑素细胞酪氨酸酶活性及黑素生成的影响

解士海, 陈志强, 卜今, 高建明, 周武庆, 李玲珺, 马鹏程   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042
  • 收稿日期:2006-01-11 出版日期:2006-11-15 发布日期:2006-11-15
  • 通讯作者: 马鹏程, email:mpc815@163.com E-mail:email;mpc815@163.com
  • 基金资助:
    江苏省自然科学基金资助项目(BK2003016)

Effects of paeonol on the tyrosinase activity and melanogenesis in co-culture model of human melanocytes and keratinocytes

XIE Shi-hai, CHEN Zhi-qiang, BU Jin, GAO Jian-ming, ZHOU Wu-qing, LI Ling-jun, MA Peng-cheng   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China
  • Received:2006-01-11 Online:2006-11-15 Published:2006-11-15

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摘要： 目的探讨丹皮酚对人表皮黑素细胞与角质形成细胞共培养体系酪氨酸酶活性及黑素生成的影响。方法培养人表皮黑素细胞及人表皮黑素细胞与角质形成细胞共培养体系,利用四甲基偶氮唑蓝(MTT)还原法测定细胞活力,采用酶学方法测定酪氨酸酶活性,475nm比色法测定黑素含量。结果50,100和200μmol/L丹皮酚对于黑素细胞及共培养细胞酪氨酸酶活性及黑素生成均有较强的剂量相关性抑制作用,100及200μmol/L丹皮酚与对照组相比差异有统计学意义(P<0.01)。结论丹皮酚对于共培养体系酪氨酸酶活性及黑素生成有较强的剂量相关性抑制作用。

关键词: 丹皮酚, 黑素细胞, 角蛋白细胞

Abstract: Objective To study the effects of paeonol on the tyrosinase activity and melanogenesis in co-culture model of human melanocytes and keratinocytes.Methods Melanocytes and the co-culture model of human melanocytes and keratinocytes were cultivated and the proliferation of melanocytes and the co-cultures was measured by MTT colorimetric assay.The tyrosinase activity and melanin level were measured by enzymic method.Results The melanin synthesis and tyrosinase activity were markedly suppressed by paeonol in a dose-dependent manner at the concentrations of 50μmol/L,100μmol/L,and 200μmol/L in both melanocytes and co-cultures.The significant stronger suppression was observed with 100μmol/L and 200μmol/L of paeonol than that with controls (P< 0.01).Conclusion Paeonol exhibits dose-dependent inhibitory effects on melanin synthesis and tyrosinase activity in co-cultures of human melanocytes and keratinocytes.

Key words: Paeonol, Melanocyte, Keratinocytes