中华皮肤科杂志

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咪喹莫特对瘢痕疙瘩成纤维细胞增殖和胶原产生的影响

董达科, 陈晓栋, 王建力   

  1. 南通大学附属医院皮肤性病科, 226001
  • 收稿日期:2005-12-12 出版日期:2006-04-15 发布日期:2006-04-15
  • 通讯作者: 陈晓栋.email:dermatochen@263.net E-mail:dermatochen@263.net

Effect of imiquimod on the proliferation of fibroblasts and production of collagen in keloid

DONG Da-ke, CHEN Xiao-dong, WANG Jian-li   

  1. Affiliated Hospital of Nantong University, Nantong 226001, China
  • Received:2005-12-12 Online:2006-04-15 Published:2006-04-15

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摘要： 目的 探讨咪喹莫特对瘢痕疙瘩成纤维细胞增殖和胶原产生的影响.方法 从手术切除的瘢痕疙瘩组织中培养成纤维细胞,加入不同浓度的咪喹莫特作用后,观察细胞的形态学变化,MTT法检测细胞的活性.免疫组化和蛋白免疫印迹法检测咪喹莫特对瘢痕疙瘩成纤维细胞Ⅰ型、Ⅲ型前胶原产生的影响.结果 在10～100μg/mL范围内,咪喹莫特能显著抑制瘢痕疙瘩成纤维细胞的增殖,且存在剂量和时间依赖性;进一步检测到咪喹莫特作用后瘢痕疙瘩成纤维细胞Ⅰ型、Ⅲ型前胶原表达减弱.结论 咪喹莫特能有效抑制瘢痕疙瘩成纤维细胞的增殖和Ⅰ型、Ⅲ型前胶原的产生.

关键词: 瘢痕疙瘩, 成纤维细胞, 胶原Ⅰ型, 胶原Ⅲ型, 咪喹莫特

Abstract: Objective To investigate the effect of imiquimod on the proliferation of fibrodblasts and production of collagen in keloid.Methods Biopsy samples of keloid were obtained from patients and cultured in vitro.Fibroblasts were harvested and passaged,treated with varying concentrations of imiquimod during the exponential phase of growth.Morphologic changes of the cells were observed,and cellular activity was determined by the MTT method.The production of procollagen typeⅠand Ⅲ in vitro was measured by immunohistochemistry and Western blot analysis.Results Imiquimod at 10μg/mL～100μg/mL could significantly inhibit the proliferation of fibroblasts in vitro in a doseand time-dependent manner.The production of procollagen typeⅠand type Ⅲ was attenuated in the presence of imiquimod.Conclusion Imiquimod can effectively inhibit the proliferation of fibroblasts and the production of procollagen typeⅠand type Ⅲ in vitro.

Key words: Fibroblasts, Collagen typeⅠ, Collagen type Ⅲ, Imiquimod, Keloid