中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (9): 575-577.

• 论著 • 上一篇    下一篇

羟氯喹及没食子酸酯对HaCaT细胞光照射的影响

闵玮, 骆丹, 林向飞, 吴迪, 缪旭   

  1. 南京医科大学第一附属医院皮肤科, 210029
  • 收稿日期:2004-09-22 发布日期:2005-09-15
  • 基金资助:
    国家自然科学基金(30371294)

Study on Photo-protective Mechanisms of Hydroxychloroquine and Epigallocatechin Gallat on Keratinocytes Damaged from Ultraviolet-B Irradiation

MIN Wei, LUO Dan, LIN Xiang-fei, WU Die, MIAO Xu   

  1. Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China
  • Received:2004-09-22 Published:2005-09-15

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摘要： 目的 探讨羟氯喹和绿茶活性成分表没食子儿茶素没食子酸酯(EGCG)对中波紫外线(UVB)损伤永生化角质形成细胞株(HaCaT细胞)的保护作用及其机制。方法 采用UVB定时及定量照射培养的HaCaT细胞,分别加入羟氯喹和EGCG干预处理,以RT-PCR法检测各受试组p53、p21、c-fos基因表达水平。结果 UVB照射后可明显增加HaCaT细胞中p53,p21,c-fos mRNA表达,羟氯喹和EGCG可不同程度地下调上述基因表达水平。结论 羟氯喹和EGCG的光保护作用在HaCaT细胞可能与其抑制p53,p21,c-fos基因表达有关。

关键词: 紫外线, 细胞, 培养的, 羟氯喹, 蛋白质P53, 原癌基因蛋白质C-FOS, 原癌基因蛋白质p21(ras), 表没食子儿茶素没食子酸酯

Abstract: Objective To investigate the photo-protective mechanisms of hydroxychloroquine and epigallocatechin gallate (EGCG) on HaCaT cells damaged from UVB irradiation. Methods Subconfluent HaCaT cells were irradiated with different doses of UVB irradiation and treated with the above listed agents. The mRNA expression levels of p53, p21, c-fos and GADPH genes were evaluated by reverse transcription-polymerase chain reaction (RT-PCR). Results UVB irradiation induced mRNA expression of p53, p21 and c-fos in cultured HaCaT cells, which were alleviated by hydroxychloroquine and EGCG treatment in UVB irradiation group. Conclusions The photo-protective effects of hydroxychloroquine and EGCG on HaCaT cells by UVB irradiation might be related to inhibition of the expression of p53,p21 and c-fos genes.

Key words: Ultraviolet rays, Cells, cultured, Hydroxychloroqine, Protein p53, Proto-oncogene proteins c-fos, Proto-oncogene proteins p21(ras), Epigallocatechin gallate