中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (9): 565-568.

• 论著 • 上一篇    下一篇

芦荟甙对中波紫外线辐射HaCaT细胞表达诱导型一氧化氮合酶及核因子κB的影响

宋秀祖1, 许爱娥1, 毕志刚2   

  1. 1. 杭州市第三人民医院皮肤科, 310009;
    2. 南京医科大学第一附属医院
  • 收稿日期:2004-10-30 发布日期:2005-09-15
  • 基金资助:
    国家自然科学基金(30271195)

Effect of Aloin on Inducible Nitric Oxide Synthase and Nuclear Factor Kappa B Synthesis of HaCat Cells Irradiated by Ultraviolet B

SONG Xiu-zu1, XU Ai-e1, BI Zhi-gang2   

  1. Department of Dermatology, The Third Hospital, Hangzhou 310009, China
  • Received:2004-10-30 Published:2005-09-15

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摘要： 目的 探讨芦荟甙对中波紫外线(UVB)辐射HaCaT细胞诱导核因子kB(NF-KB)及诱导型一氧化氮合酶(iNOS)表达的抑制作用。方法 采用MTT法测定HaCaT细胞的增殖变化,生化比色法检测培养细胞上清液中NO含量,RT-PCR法检测HaCaT细胞诱导型一氧化氮合酶(iNOS)mRNA的表达变化,免疫荧光细胞化学染色测定NF-kB P65的激活表达。结果 30 mJ/cm2 UVB辐射HaCaT细胞,细胞的增殖明显下降,NO的合成分泌增加,iNOS mRNA表达显著增加,同时NF-kB被激活,从细胞浆易位到细胞核。芦荟甙对HaCaT细胞的增殖有显著促进作用。用不同浓度芦荟甙预处理HaCaT细胞,可以显著抑制UVB辐射诱导的NF-kB激活,下调iNOS mRNA表达及NO合成分泌。经统计学分析,差异有统计学意义(P<0.01)。结论 芦荟甙通过抑制UVB辐射诱导的NF-kB P65激活,下调iNOS mRNA表达,减少NO合成分泌,发挥防护紫外线辐射损伤的作用,在炎症性皮肤病防治中可能发挥重要作用。

关键词: 紫外线, 细胞, 培养的, 一氧化氮合酶, NF-κB, 芦荟甙

Abstract: Objective To investigate the protective effect of aloin on inducible nitric oxide synthase (iNOS) and nuclear factor kappa B (NF-kB) synthesis of HaCat cells induced by ultraviolet B (UVB) irradiation. Methods The proliferation of HaCat cells was measured by MTT method. iNOS mRNA was detected by reverse transcriptase polymerase chain reaction (RT-PCR). NO production was assessed by spectrophotometric method, and expression of NF-kB P65 was measured by immunofluorescent staining. Results After irradiation with 30 mJ/cm2 of UVB, proliferation of HaCat cells was decreased, and NO generation and iNOS mRNA synthesis in HaCat cells were increased. UVB irradiation could also activate the expression of NF-kB P65 and promote its translocation into nucleus. NO generation and iNOS mRNA synthesis were markedly down-regulated in a dose-dependent manner by pre-treatment with different concentrations of aloin. The activation of NF-kB P65 was inhibited while the proliferation of HaCat cells was increased. All the difference reached statistical significance (P<0.01). Conclusions Our Results confirm that aloin treatment could inhibit NF-kB P65 activation, and down-regulate iNOS mRNA expression and NO generation induced by UVB irradiation. Aloin might play an important role in the treatment of skin inflammatory diseases, especially ultraviolet irradiation injury.

Key words: Ultraviolet rays, Cells, cultured, Nitric-oxide synthase, NF-kappa B, Aloin