中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (9): 557-559.

• 论著 • 上一篇    下一篇

甘草甜素对小鼠朗格汉斯细胞的影响

郭哲, 高兴华, 王雅坤, 陈洪铎   

  1. 中国医科大学附属第一医院皮肤科, 沈阳110001
  • 收稿日期:2005-03-14 发布日期:2005-09-15

The Effects of Glycyrrhizin on Langerhans' Cells of Mice

GUO Zhe, GAO Xing-hua, WANG Ya-kun, CHEN Hong-duo   

  1. Department of Dermatology, No.1 Hospital of China Medical University, Shenyang 110001, China
  • Received:2005-03-14 Published:2005-09-15

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摘要： 目的 探讨甘草甜素对小鼠皮肤朗格汉斯细胞的影响。方法 甘草甜素、生理盐水和地塞米松每日1次腹腔注射后在不同时间点获取耳部皮肤。用免疫组化技术检测小鼠表皮中的朗格汉斯细胞。结果 ①甘草甜素5 mg/kg、10 mg/kg及地塞米松1 mg/kg用药3 d后和1周后与用药0 d相比,朗格汉斯细胞密度显著降低。②甘草甜素80 mg/kg用药3 d后和1周后与用药0 d相比,朗格汉斯细胞密度显著升高。③用药4周后甘草甜素对朗格汉斯细胞密度的影响渐弱。结论 甘草甜素对朗格汉斯细胞密度具有双相调节作用。低剂量使朗格汉斯细胞密度降低,高剂量使朗格汉斯细胞密度升高。

关键词: 甘草甜素, 郎格尔汉斯细胞

Abstract: Objective To explore the effects of glycyrrhizin (GL) on Langerhans cells (LCs) of mice. Methods The mice were treated intraperitoneally with normal saline, different doses of GL and dexamethasone, respectively, once daily. The mouse ear skin was excised at different times after treatment. The mouse LCs were detected by immunohistochemical technique after treatment, using rat IgG2a anti-mouse Ia monoclonal antibody. Results After 3 and 7 days of treatment, the densities of LC were significantly decreased in the groups treated with 5 mg/kg, 10 mg/kg of GL and 1 mg/kg of dexamethasone, but significantly increased in group treated with 80 mg/kg of GL. The effects of GL on the density of mouse LC weakened after 4 weeks of treatment. Conclusions The densities of mouse LC, decreased with low dose but increased with high dose of GL treatment, can be regulated in both directions by GL.

Key words: Glycyrrhizin, Langerhans cells