中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (8): 509-511.

• 论著 • 上一篇    下一篇

Toll样受体4在小鼠系统性白念珠菌感染中的作用

陈兴平, 熊瑛, 黄朝卫, 陈映玲   

  1. 华中科技大学同济医学院附属同济医院皮肤科 武汉 430030
  • 收稿日期:2004-10-08 出版日期:2005-08-15 发布日期:2005-08-15

The Role of Toll-like Receptor 4 in Systemic Candidiasis in Murine Model

CHEN Xing-ping, XIONG Ying, HUANG Chao-wei, CHEN Ying-ling   

  1. Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong Science & Technology University, Wuhan 430030, China
  • Received:2004-10-08 Online:2005-08-15 Published:2005-08-15

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摘要： 目的 探讨Toll样受体4(TLR4)在小鼠系统性白念珠菌感染中的作用.方法 ①建立环磷酰胺诱导的免疫抑制小鼠系统性白念珠菌感染动物模型,设置实验组,即C3H/HeJ组(TLR4基因突变),及对照组,即C3H/HeN组(TLR4正常).②用平皿稀释法检测肾脏、脾脏组织菌落形成单位(cfu).③制作肾脏组织病理学标本PAS染色,评估其真菌感染程度.④酶联免疫吸附试验检测肾脏组织肿瘤坏死因子α分泌水平.结果 ①实验组感染后第1、6天,肾脏cfu值明显高于对照组;感染后第1天,实验组脾脏cfu值亦明显高于对照组,差异有统计学意义.②感染后第1、6天,实验组肾脏真菌感染程度评分高于对照组,差异有统计学意义.③感染后第1、6天,实验组肾脏分泌肿瘤坏死因子α水平低于对照组,且有统计学意义.结论 TLR4在宿主抗系统性白念珠菌感染中发挥保护作用.

关键词: Toll样受体4, 念珠菌病, 动物替代试验

Abstract: Objective To investigate the role of Toll-like receptor 4 (TLR4) in the host defense against systemic candidiasis in murine model.Methods Two groups of mice,a study group (C3H/HeJ mice with mutant TLR4 gene) and a control group (C3H/HeN mice with normal TLR4 gene) were set up in cyclophosphamide-induced immuno-suppressed murine model with systemic candidiasis.Colony forming units (CFUs) of C.albicans were determined in infected kidneys and spleens with plating dilution method.Histopathological changes of infected kidneys were measured.In addition,levels of tumor necrosis factor-α (TNF-α) in kidneys were detected by enzyme-linked immunosorbent assay.Results CFUs of C.albicans were significantly higher in kidneys in the 1st day and 6th day after infection in the study group than those in the control group.Meanwhile,CFUs of C.albicans were significantly higher in spleens in the 1st day after infection in the study group than those in the control group.The scores of severity of infection,showed by histopathology,were significantly higher in kidneys in the 1st day and 6th day after infection in the study group than those in the control group(P<0.05).TNF-α concentrations were significantly lower in kidneys in the 1st day and 6th day after infection in the study group than those in the control group (P<0.05).Conclusion TLR4 may play a protective role in host defense against systemic candidiasis in murine model.

Key words: Toll-like receptor, Candidiasis, Animal testing alternatives