白念珠菌对人血管内皮细胞表面黏附分子表达和细胞因子分泌的影响

摘要/Abstract

摘要： 目的观察白念珠菌对人血管内皮细胞表面细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)表达及分泌白介素6(IL-6)、白介素8(IL-8)水平的影响.方法分离健康产妇胎盘脐静脉内皮细胞进行原代培养,细胞长至单层时,与白念珠菌共培养不同时间,再用逆转录-聚合酶链反应法分析内皮细胞表面ICAM-1mRNA、VCAM-1mRNA的表达,用ELISA法检测IL-6、IL-8分泌水平.结果白念珠菌刺激4h后血管内皮细胞表达ICAM-1 mRNA、VCAM-1 mRNA明显增加,至8h达到峰值.而IL-6,IL-8的分泌在4h后也明显增加(P<0.05),24h到达高峰(P<0.01).结论白念珠菌可诱导人血管内皮细胞ICAM-1 mRNA、VCAM-1 mRNA表达及促进IL-6、IL-8分泌.

关键词: 念珠菌,白色, 内皮,血管, 胞间黏附分子1, 血管细胞黏附分子-1, 白细胞介素6, 白细胞介素8

Abstract: Objective To investigate the expression of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1(VCAM-1) on the surface of human vascular endothelial cells (HUVECs) stimulated by Candida albicans.Methods HUVECs were isolated from the umbilical vein of placenta of healthy parturients and cultured.The primary culture of HUVECs was stimulated with Candida albicans at different intervals.Expression of ICAM-1 and VCAM-1 mRNA were measured by reverse transcription-PCR,and the levels of secretion of IL-6 and IL-8 by enzyme-linked immunosorbent assay(ELISA).Results ICAM-1 and VCAM-1 mRNA expression in HUVECs began to increase after stimulation with C.albicans in 4 h and reached peak level in 8 h.Secretion of IL-6 and IL-8 by HUVECs began to increase after stimulation by C.albicans in 4 h and reached peak level in 24 h.Conclusion C.albicans could induce expression of ICAM-1 and VCAM-1 mRNA in HUVECs,and enhance secretion of IL-6 and IL-8 by HUVECs.

Key words: Candida albicans, Endothelium,vascular, Intercellular adhesion molecule-1, Vascular cell adhesion molecule-1, Interleukin-6, Interleukin-8

江文, 万沐芬, 陈兴平. 白念珠菌对人血管内皮细胞表面黏附分子表达和细胞因子分泌的影响[J]. 中华皮肤科杂志, 2005, 38(8): 506-508.doi:

JIANG Wen, WAN Mu-fen, CHEN Xing-ping. Effects of Candida albicans on Expression of Adhesion Molecules and Secretion of Cytokines by Human Vascular Endothelial Cells[J]. Chinese Journal of Dermatology, 2005, 38(8): 506-508.doi:

参考文献

[1] Klotz SA. Fungal adherence to the vascular compartment:a critical step in the pathogenesis of disseminated candidiasis. Clin Infect Dis, 1992, 14:340-347.
[2] Jaffe EA. Culture of human endothelial cells derived from human umbillical veins. Circulation, 1972, 46:211-253.
[3] Filler SG, Pfunder AS, Spellberg BJ, et al. Candida albicans stimulates cytokine production and leukocyte adhesion molecule expression by endothelial cells. Infect Immun, 1996, 64:2609-2617.
[4] Couffinhal T, Duplaa C, Moreau C, et al. Regulation of vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 in human vascular smooth muscle cells. Circ Res, 1994, 74:225-234.
[5] Ishizuka T, Kawakami M, Hidaka T, et al. Stimulation with thromboxane A2 (TXA2) receptor agonist enhances ICAM-1, VCAM-1 or ELAM-1 expression by human vascular endothelial cells. Clin Exp Immunol, 1998, 112:464-470.
[6] Lawrence MB, Springer TA. Leukocytes roll on a selectin at physiologic flow rates:distinction from and prerequisite for adhesion through integrins. Cell, 1991, 65:859-873.
[7] Osborn L, Hession C, Tizard R, et al. Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that binds to lymphocytes. Cell, 1989, 59:1203-1211.
[8] Lotz M, Jirik F, Kabouridis P, et al. B cell stimulating factor 2/interleukin 6 is a costimulant for human thymocytes and T lymphocytes. J Exp Med, 1988, 167:1253-1258.
[9] Shabo Y, Lotem J, Rubinstein M, et al. The myeloid blood cell differentiation-inducing protein MGI-2A is interleukin-6. Blood,1988, 72:2070-2073.
[10] Mukaida N, Harada A, Matsushima K. Interleukin-8 (IL-8) and monocyte chemotactic and activating factor (MCAF/MCP-1),chemokines essentially involved in inflammatory and immune reactions. Cytokine Growth Factor Rev, 1998, 9:9-23.

[1]	许昌春杜蕾蕾曾荣童建波刘宇甄段志敏陈旭徐浩翔龚春燕李岷. 白介素6在痤疮囊肿皮损中的表达及痤疮丙酸杆菌体外对THP-1细胞白介素6水平的影响[J]. 中华皮肤科杂志, 2018, 51(4): 265-268.
[2]	尧志建王梅王香兰王煜任建文刘平王琼玉. 热休克蛋白70上调寻常性银屑病成纤维细胞白介素6表达[J]. 中华皮肤科杂志, 2012, 45(11): 792-795.
[3]	陈淑君, 方栩, 王侠生. 头皮屑中白介素1α、白介素1受体拮抗剂和白介素8的测定[J]. 中华皮肤科杂志, 2007, 40(2): 92-94.
[4]	米向斌, 谭国珍, 尹若菲, 曾凡钦. 氮杂胞苷对SLE患者外周血T细胞Th2细胞因子基因表达的影响[J]. 中华皮肤科杂志, 2007, 40(1): 22-24.
[5]	黄雯, 黄岚, 李峰, 冯树芳, 徐金华. 奥曲肽对系统性红斑狼疮患者体外培养单一核细胞增殖及分泌细胞因子的影响[J]. 中华皮肤科杂志, 2006, 39(9): 527-529.
[6]	崔盘根, 林麟, 毕志刚, 骆丹, 张福仁, 田洪清, 连石. 抗人白介素8单克隆抗体乳膏治疗湿疹的多中心随机双盲对照研究[J]. 中华皮肤科杂志, 2006, 39(7): 394-396.
[7]	尹国华, 李新华, 张开明, 刘玉峰. 银屑病患者骨髓CD34⁺细胞体外定向分化的T细胞活性研究[J]. 中华皮肤科杂志, 2006, 39(3): 124-127.
[8]	冯华, 李新宇, 郑家润. 来氟米特对地蒽酚诱导炎症因子表达的调控[J]. 中华皮肤科杂志, 2006, 39(2): 89-91.
[9]	黄雯, 冯树芳, 徐金华, 李峰. 催乳素与促生长素对体外培养系统性红斑狼疮患者单一核细胞产生细胞因子的影响[J]. 中华皮肤科杂志, 2005, 38(4): 217-219.
[10]	洪为松, 尉晓冬, 周渭珩, 许爱娥. 白癜风患者血清中抗酪氨酸酶抗体及可溶性细胞间黏附分子1的检测[J]. 中华皮肤科杂志, 2005, 38(10): 612-614.
[11]	周渭珩, 程东庆, 洪为松, 徐瑾, 许爱娥. 血清可溶性黏附分子与系统性红斑狼疮患者活动性的关系[J]. 中华皮肤科杂志, 2005, 38(1): 17-19.
[12]	姚煦, 李遇梅, 弓娟琴, 胡兹嘉, 李文忠, 陈志强. 系统性红斑狼疮患者外周血单一核细胞白介素6受体水平的研究[J]. 中华皮肤科杂志, 2005, 38(1): 20-22.
[13]	郑文军, 林有坤, 韦凤鲜, 覃伟武, 罗佐杰, 潘尚领. 慢性荨麻疹患者甲状腺功能的研究[J]. 中华皮肤科杂志, 2004, 37(2): 103-104.
[14]	农祥, 何黎, 万屏, 邹勇莉, 刘玲, 郭晋. 昆明山海棠抗接触变应性光皮炎的研究[J]. 中华皮肤科杂志, 2004, 37(10): 592-594.
[15]	顾劲松, 涂彩霞, 谭雪晶, 谷玲, 林熙然. 白癜风患者血清白介素、肿瘤坏死因子α及粒细胞-巨噬细胞集落刺激因子检测[J]. 中华皮肤科杂志, 2003, 36(2): 73-75.