中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (6): 377-379.

• 论著 • 上一篇    下一篇

天然抗角蛋白单克隆抗体3B4的多反应性鉴定及其分子机制的初步探讨

管海宏, 付萌, 夏汝山, 王刚, 刘玉峰   

  1. 第四军医大学西京医院皮肤科 西安 710032
  • 收稿日期:2004-06-24 出版日期:2005-06-15 发布日期:2005-06-15
  • 基金资助:
    国家自然科学基金资助项目(30330510);军队重点基金资助项目(01Z086)

Identification of the Polyreactivity of Monoclonal Natural Anti-ker atin Autoantibody 3B4 and Analysis of Its Molecular Mechanism

GUAN Hai-hong, FU Meng, XIA Ru-shan, WANG Gang, LIU Yu-feng   

  1. Department of Dermatology, Xijing Hospital, the Forth Military Medical University, Xi'an 710032, China
  • Received:2004-06-24 Online:2005-06-15 Published:2005-06-15

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摘要： 目的 对天然抗角蛋白单克隆抗体3B4的多反应性进行鉴定,并探讨其分子基础。方法 用间接酶联免疫吸附实验(ELISA)和免疫组化法检测3B4与多种抗原的反应活性,分析其可变区基因与氨基酸序列。结果 3B4除与角蛋白和皮肤发生较强的反应外,尚能选择性与多种具有不同表位的抗原和不同的组织反应;同源性分析表明,其重、轻链可变区基因分别与胚系基因VH1 J558.42及VK1 am4高度同源;HCDR3区为该抗体区别于其他同源性较高的免疫球蛋白的惟一基序,主要由短的侧链氨基酸组成。结论 天然抗角蛋白单克隆抗体3B4具有多反应性,由少量突变的胚系基因编码,HCDR3区的柔韧性可能是多反应性的分子基础之一。

关键词: 自身抗体, 角蛋白, 抗体特异性

Abstract: Objective To identify the polyreactivity of a monoclonal natural anti-keratin autoantibody 3B4 and to analyze its possible molecularme chanism. Methods enzyme-linked immunosorbent assay (ELISA)and immunohistoche mistry were applied to test the binding reactivity of 3B4 against different antigens and tissues.The variable region genes and their aminoacid composition were sequenced. Results 3B4 could reacted with a range of antigens and tissues,in addition to keratin and skin.The variable region genes of its light chain and heavy chain showed high homology with germline genes VK1 am4 and VH1 J558.42.H CDR3 region,which mainly composed of short side chain amino acids(from 294 to 324 nucleotides around the heavy chain),was the only motif that differs from ot her highly homologous immunoglobulin genes. Conclusions The monoclonal natural anti-keratin autoantibody 3B4,with its variable region genes highly homologo us to germline genes,is highly polyreactive.The flexibility of HCDR3 may contr ibute to the polyreactivity.

Key words: Autoantibodies, Keratin, Antibody specificity