中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (10): 628-630.

• 论著 • 上一篇    下一篇

Tcl/Tc2失衡和慢性光线性皮炎发病的相关性研究

陈浩1, 邓丹琪1, 周晓鸿1, 樊应俊1, 杨红英2   

  1. 1. 昆明医学院第二附属医院皮肤性病科 650101;
    2. 昆明医学院第二附属医院检验科 650101
  • 收稿日期:2004-10-13 发布日期:2005-10-15
  • 通讯作者: 邓丹琪,E-mail:danqid@hotmail.com E-mail:danqid@hotmail.com
  • 基金资助:
    国家自然科学基金(30260094)

Imbalance of Tc Cells in the Pathogenesis of Chronic Actinic Dermatitis

CHEN Hao1, DENG Dan-qi1, ZHOU Xiao-hong1, FAN Ying-jun1, YANG Hong-ying2   

  1. Department of Dermatology, The Second Affiliated Hospital of Kunming Medical College, Kunming 650101, China
  • Received:2004-10-13 Published:2005-10-15

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摘要： 目的 探讨Tc细胞失衡和慢性光线性皮炎(CAD)发病的相关性。方法 应用免疫组化SP法检测28例CAD患者和7例正常人对照者皮肤中真皮浸润细胞的免疫表型及白介素4、10、12,干扰素γ受体的表达。同时应用ELISA的方法检测33例CAD和32例正常人对照者血清中白介素4、10、12、干扰素γ的含量。结果 CAD患者皮损真皮内浸润的单一核细胞绝大部分表达为CD3+阳性的T细胞,阳性细胞百分数,平均灰度与CD20+阳性的B细胞相比有统计学意义(P均<0.001)。这些T细胞中大部分表达为CD8+阳性的T细胞,阳性细胞百分数和平均灰度与CD4+阳性的T细胞相比有统计学意义(P均<0.001)。Tcl细胞分泌干扰素γ,白介素12的受体在CAD患者真皮内浸润的Tc细胞表面的表达要高于Tc2细胞分泌的白介素10、4的受体表达(P均<0.001)。外周血干扰素γ、白介素12含量在CAD患者组和对照组中有统计学意义(P均<0.05)。结论 CAD发展可能和Tcl细胞的过度表达及Tc2细胞的表达受抑有一定的关系。

关键词: 光过敏疾患, T淋巴细胞, 辅助诱导, T淋巴细胞, 细胞毒性, 白细胞介素类, 干扰素Ⅱ型

Abstract: Objective To investigate the association of imbalance of Tc1 and Tc2 cells with chronic actinic dermatitis (CAD). Methods Immunohistochemistry (S-P method) was used to test the subgroups of infiltrating cells and the expression of receptors of interleukin-4 (IL-4), interleukin-10 (IL-10), interleukin-12 (IL-12) and interferon-γ (IFN-γ) in these cells of the eruptions from 28 patients with CAD and 7 normal controls. The serum levels of IL-4, IL-10, IL-12 and IFN-γ from 33 patients with CAD and 32 normal controls were tested by ELISA. Results The major infiltrating cells in the eruptions of patients with CAD were T cells, which were significantly more than B cells (P<0.001). Among these T cells, the predominant was Tc cells, which were significantly more than Th cells (P<0.001). In the ease of infiltrating cells, the expression of receptors of IL-12 and IFN-γ was significantly higher than that of receptors of IL-4 and IL—10 (P<0.001). The serum levels of IL-12 and IFN-γ in patients with CAD were significantly increased than those in the control group (both P<0.05). Conclusions These findings indicate the imbalance of Tc1 and Tc2 cells probably plays an important role in the etiology and pathogenesis of CAD.

Key words: Photosensitivity disorders, T lymphocytes, helper-inducer, T lymphocytes, eytotoxic, Interleukins, Interferon typeⅡ