中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (6): 351-353.

• 论著 • 上一篇    下一篇

慢性荨麻疹患者血清组胺释放活性检测

孙蔚凌, 毕志刚   

  1. 南京医科大学第一附属医院皮肤科 210029
  • 收稿日期:2003-06-04 出版日期:2004-06-15 发布日期:2004-06-15

Evaluation of Histamine-releasing Activity in the Sera of Patients with Chronic Urticaria

SUN Wei-ling, BI Zhi-gang   

  1. Department of Dermatology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China
  • Received:2003-06-04 Online:2004-06-15 Published:2004-06-15

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摘要： 目的 检测慢性荨麻疹患者血清组胺释放活性,探讨慢性荨麻疹的发病机制.方法 通过体外分离人皮肤肥大细胞,进行肥大细胞组胺释放试验,测定组胺释放率.结果 62例慢性荨麻疹患者中,自体血清皮肤试验阳性者24例占38.71%.混合细胞悬液中肥大细胞的组胺自发释放率<5%.血清活化皮肤肥大细胞引起的组胺释放率从3.1%～79.5%(16.44%±14.26%),明显高于正常人对照组(P<0.01),其中27例组胺释放率>15%(43.55%);自体血清皮肤试验(+)组的组胺释放率及阳性率均明显高于自体血清皮肤试验(-)组(P<0.01).结论 部分慢性荨麻疹患者血清中存在组胺释放活性,可直接活化肥大细胞,释放组胺等血管活性介质,引起荨麻疹.

关键词: 荨麻疹, 肥大细胞, 组胺释放, 皮肤试验

Abstract: Objective To evaluate the serum histamine-releasing activity of patients with chronic urticaria and explore the pathogenesis of chronic urticaria. Methods Sixty-two chronic urticaria patients were studied by using in vivo autologous serum skin test (ASST) and in vitro histamine release assay of human cutaneous mast cells incubated with the patient's sera. Results Twenty-four out of 62 patients showed a wheal response to autologous serum (38.71%). Sera from patients with chronic urticaria released a significantly higher quantity of histamine compared with the control subjects (P<0.01), the percentage of histamine release varied from 3.1% to 79.5% (16.44%±14.26%). Twenty-seven serum samples (43.55%) elicited histamine release higher than 15%. Compared with the ASST negative patients, the positive rate and percentage of histamine release were also elevated in the ASST positive patients (P<0.01). Conclusion Histamine-releasing activity is important in the pathogenesis of chronic urticaria by stimulating or facilitating degranulation of cutaneous mast cells.

Key words: Urticaria, Mast cells, Histamine release, Skin tests