中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (6): 329-331.

• 论著 • 上一篇    下一篇

紫外线灭活的EB病毒蛋白对抗角蛋白自身抗体产生的调节作用

张衍国1, 付萌1, 刘玉峰1, 高天文1, 潘蕾2, 王琳1   

  1. 1. 第四军医大学西京医院全军皮肤性病中心 西安 710032;
    2. 第四军医大学唐都医院传染科
  • 收稿日期:2003-11-03 出版日期:2004-06-15 发布日期:2004-06-15
  • 基金资助:
    国家自然科学基金资助(30170862)

UV-inactivated Epstein-Barr Virus Protein Regulating the Production of Antikeratin Autoantibodies

ZHANG Yan-guo1, FU Meng1, LIU Yu-feng1, GAO Tian-wen1, PAN Lei2, WANG Lin1   

  1. Center of Dermatology of Chinese PLA, Xijing Hospital, Fourth Military Medical University, Xian, Shanxi Province 710032, China
  • Received:2003-11-03 Online:2004-06-15 Published:2004-06-15

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摘要： 目的 研究紫外线(UV)灭活和热处理的EB病毒刺激培养的人脐带血B细胞产生抗角蛋白自身抗体(AKautoAb)的作用.方法 用淋巴细胞分离液分离人脐带血单一核细胞,L-亮氨酸甲酯去除单核细胞、NK细胞和细胞毒性T细胞,2-氨乙基硫脲溴化物处理的绵羊红细胞去除T细胞,从而获得纯化B细胞.用UV灭活和热处理EBV处理培养的B细胞.流式细胞仪检测UV灭活EBV组细胞CD5、CD3、CD4和CD8的表达.ELISA法检测培养上清液中AK auto Ab IgG和IgM的产生,并与EBV转化B细胞产生的AKautoAbIgG和IgM作对比.结果 细胞培养14d时,未检测到T细胞,CD5+B细胞占43%;28d时,CD5+B细胞占47%.UV灭活EBV组AK auto Ab IgG1 8d以后各时间点有显著变化(P<0.05),AK auto Ab IgM 26d与其他时间点差异有显著性(P<0.05).热处理EBV组AK auto Ab IgG和IgM无明显变化(P>0.05).EBV转化B细胞40d,AK auto Ab IgG和IgM的产生与同期的UV灭活EBV组差异有显著性(P<0.05).结论 UV灭活EBV有诱导AK auto Ab产生的作用,而热处理EBV不能诱导抗体的产生,提示灭活EBV诱导AK auto Ab产生可能是通过EBV的蛋白成分实现的.

关键词: 角蛋白, 自身抗体, 疱疹病毒4型,人, B淋巴细胞

Abstract: Objective To investigate UV-or heat-inactivated Epstein-Barr virus(EBV)stimulating the production of anti-keratin autoantibody(AK auto Ab)in cultured human umbilical cord blood B cells. Methods Mononuclear cells were isolated routinely from umbilical cord blood, in which monocytes, NK cells and cytotoxicity T cells were eliminated by L-leucine methyl ester method, and T cells were removed by sheep red blood cells(SRBCs)treated with 2-amino ethyl-isothiouronium bromide (AET). The purified B cells were treated with UV-or heat-inactivated EBV respectively and then cultured in complete IMDM. CD5, CD3, CD4 and CD8 cells were detected by flow cytometry. IgG and IgM of AK auto Ab were measured by ELISA in the supernatant which came from the B cells treated by UV-inactivated EBV or EBV-transformed B cells respectively. Results In UV-inactivated EBV group CD5+B cells accounted for 43% and 47% of all cells detected on the 14th and 28th day, respectively. No CD3, CD4 and CD8 cells were detected during this period. In UV-inactivated EBV group the AK auto Ab of IgG and IgM increased significantly on the 18th and 26th day, respectively (P<0.05, P<0.05), but no significant difference in heat-inactivated EBV group (P>0.05). On the 40th day the AK auto Ab of IgG and IgM were significantly higher in EBV-transformed B cell group than those in UV-inactivated EBV group. Conclusions UV-inactivated EBV is able to induce AK auto Ab production but heat-inactivated EBV does not, which suggests that EBV protein might be the effective agent in inducing the production of AK auto Ab.

Key words: Keratin, Autoantibodies, Herpesvirus 4, human, B-lymphocytes