中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (4): 218-220.

• 论著 • 上一篇    下一篇

三氧化二砷对小鼠恶性黑素瘤细胞Cloudman S91的作用

侯麦花, 朱文元, 卢新政   

  1. 南京医科大学第一附属医院皮肤科 210029
  • 收稿日期:2003-04-16 出版日期:2004-04-15 发布日期:2004-04-15
  • 基金资助:
    国家自然科学基金资助项目(30170861)

Effects of Arsenic Trioxide on Mouse Cultured Melanoma Cloudman S91 Cells

HOU Mai-hua, ZHU Wen-yuan, LU Xin-zheng   

  1. Department of Dermatology, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
  • Received:2003-04-16 Online:2004-04-15 Published:2004-04-15

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摘要： 目的 观察不同浓度三氧化二砷(As2O3)对体外培养小鼠黑素瘤Cloudman S91(S91)细胞的影响.方法 以不同浓度As2O3诱导体外培养小鼠S91黑素瘤细胞24、48、72、96、120h,用四氮唑(MTT)还原法检测细胞增殖;观察细胞病理组织学变化;计数其集落形成;流式细胞仪测定细胞周期及凋亡率.结果 0.031~0.25μmol/L As2O3促进小鼠黑素瘤细胞增殖及集落形成,诱导14d后大多数细胞处于G0-G1期,胞核变小,核浆比例增大,核仁减少.0.5~8μmol/LAs2O显著抑制小鼠S91细胞增3殖,2~8μmol/L As2O3明显促进细胞凋亡.结论 As2O3明显影响小鼠S91细胞增殖、分化及生长周期及凋亡,并呈浓度及时间依赖性.低浓度时促进其增殖,并有一定诱导细胞分化作用,高浓度时显著抑制其增殖,并促进部分细胞凋亡.

关键词: 砷剂, 黑色素瘤,实验性, 细胞凋亡

Abstract: Objectives To observe the effects of arsenic trioxide (As2O3) on the cell cycle, differentiation and proliferation of cultured mouse melanoma Cloudman S91 cells. Methods The cell viability was measured by MTT reduction assay. The morphological changes of the cells were examined by Wright-Giemsa staining. Clone forming was carried out to test the proliferation ability. Apoptosis rate and cell cycle of S91 cells were measured by flow cytometry. Results As2O3 in the concentrations of 0.031~0.25 μmol/L promoted cell growth markedly and blocked the transformation of cells from G0 to G1. As2O3 in the concentrations of 0.5~8.0 μmol/L inhibited the cell growth and induced apoptosis markedly. Conclusions As2O3 has marked effects on mouse melanoma S91 cells, which include inducing apoptosis and promoting differentiation and proliferation. These effects are dose- and time-dependent.

Key words: Arsenicals, Melanoma, experimental, Apoptosis