积雪甙对黑素瘤细胞生长影响的实验研究

中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (2): 71-73.

积雪甙对黑素瘤细胞生长影响的实验研究

桑红, 倪容之, 沈献平, 叶楠, 曹扬, 张敏, 倪通, 谭立恒

南京军区南京总医院皮肤科 210002

收稿日期:2003-03-15 出版日期:2004-02-15 发布日期:2004-02-15

The Effects of Asiaticoside on the Growth of Melanoma B16 Cells

SANG Hong, NI Rong-zhi, SHEN Xian-ping, YE Nan, CAO Yang, ZHANG Min, NI Tong, TAN Li-heng

Department of Dermatology, Nanjing General Hospital of Nanjing Command, Nanjing 210002, China

Received:2003-03-15 Online:2004-02-15 Published:2004-02-15

摘要/Abstract

摘要： 目的研究积雪甙对黑素瘤细胞生长的影响。方法将黑素瘤细胞B16常规传代培养,取其生长状态良好的对数生长期细胞,观察积雪甙对B16细胞的生长抑制作用,并进行对B16细胞生长抑制作用的形态学观察,用流式细胞仪检测积雪甙对B16细胞诱导凋亡的作用。结果随着积雪甙的剂量增加,对B16细胞有丝分裂的抑制增加。代表凋亡早期细胞的annexin-v阳性细胞比例增加,摄取标记细胞线粒体的R123的细胞比例降低,而标记核内DNA的PI阳性细胞的比例增加,表明该药能够诱导细胞凋亡或致死亡。结论积雪甙有抗黑素瘤细胞生长作用。

关键词: 细胞凋亡, 肿瘤细胞,培养的, 积雪草

Abstract: Objective To study the effects of asiaticoside on the growth of melanoma B16 cell cultures in vitro. Methods Melanoma B16 cells were subcultured and the inhibition of cellular growth was investigated. The morphology of the cells was observed after inhibition. The induction of apoptosis by asiaticoside was determined by flow cytometry. Results It was found that asiaticoside could significantly inhibit the growth of B16 cell cultures in vitro in a dose-dependent manner. The annexin-v positive cells were increased, along with that cells intaking R123 marked mitochondria were decreased, and PI positive cells increased, which indicated that cellular apoptosis was induced. Conclusion Asiaticoside plays an inhibitory role in the growth of melanoma B16 cells.

Key words: Apoptosis, Tumor cells, cultured, Centella asiatica

桑红, 倪容之, 沈献平, 叶楠, 曹扬, 张敏, 倪通, 谭立恒. 积雪甙对黑素瘤细胞生长影响的实验研究[J]. 中华皮肤科杂志, 2004, 37(2): 71-73.

SANG Hong, NI Rong-zhi, SHEN Xian-ping, YE Nan, CAO Yang, ZHANG Min, NI Tong, TAN Li-heng. The Effects of Asiaticoside on the Growth of Melanoma B16 Cells[J]. Chinese Journal of Dermatology, 2004, 37(2): 71-73.

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