中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (11): 653-655.

• 论著 • 上一篇    下一篇

他克莫司影响朗格汉斯细胞迁移机制的研究

唐玲1, 于益芝2, 陈洁1, 阎明1, 顾军1, 郑茂荣1   

  1. 1. 第二军医大学附属长海医院皮肤 上海 200433;
    2. 第二军医大学免疫学研究所
  • 收稿日期:2003-11-06 出版日期:2004-11-15 发布日期:2004-11-15
  • 通讯作者: 于益芝, E-mail:cjcb@public9.sta.net.cn E-mail:cjcb@public9.sta.net.cn
  • 基金资助:
    国家自然科学基金(30271202);国家863计划(2001AA215091);上海市卫生局科技发展基金(034113)

The Mechanisms of Tcrolimus on the Migration of Langerhans'Cells

TANG Ling1, YU Yi-zhi2, CHEN Jie1, YAN Ming1, GU Jun1, ZHENG Mao-rong1   

  1. Department of Dermatology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China
  • Received:2003-11-06 Online:2004-11-15 Published:2004-11-15

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摘要： 目的 探讨他克莫司影响朗格汉斯细胞迁移能力的原因。方法 用不同浓度的他克莫司处理人角质形成细胞株HaCaT,24h后收集上清液,通过ELISA法检测上清液中的单核细胞化学吸引蛋白质1(monocytechemoattractantprotein1,MCP-1)浓度,并通过RT-PCR法检测细胞中MCP-1mRNA水平。结果 经质量浓度为625～5000ng/mL他克莫司处理过的HaCaT株,其分泌至上清液中的MCP-1水平明显受到抑制,细胞中MCP-1mRNA的水平低于正常对照组。结论 他克莫司通过抑制角质形成细胞中MCP-1的分泌、表达,进而影响了朗格汉斯细胞前体及朗格汉斯细胞趋化至皮损处,阻止或减弱了某些免疫反应。

关键词: 郎格尔汉斯细胞, 免疫抑制剂, 单核细胞化学吸引蛋白质1, 细胞运动

Abstract: Objective To investigate the mechanisms of tacrolimus on the migration of Langerhans;cells.Methods The concentration of MCP-1 in the cultured supernatants of HaCaT cell treated with tacrolimus was determined by ELISA.The expression level of MCP-1 mRNA in HaCaT cell treated with tacrolimus was studied by RT-PCR method.Results The concentration of MCP-1 in HaCaT cell treated with 625 ～ 5 000 ng/mL of tacrolimus was significantly decreased.The expression level of MCP-1 mRNA was also markedly decreased.Conclusions Tacrolimus may probably decrease the expression of MCP-1 in keratinocytes and suppress the chemotactic ability of Langerhans;cells precursor and Langerhans;cells,thus inhibiting or diminishing certain local immunoreaction.

Key words: Langerhans cells, Immunosuppressive agents, Monocyte chemoattractant protein-1, Cell movements