中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (11): 648-650.

• 论著 • 上一篇    下一篇

条件培养基对高传代人毛乳头细胞生长的影响

罗洋, 郝飞, 钟白玉, 麦跃, 姜晓勇   

  1. 第三军医大学西南医院皮肤科 重庆 400038
  • 收稿日期:2003-11-03 出版日期:2004-11-15 发布日期:2004-11-15
  • 通讯作者: 郝飞, E-mail:Haofei@mail.tmmu.com.cn E-mail:Haofei@mail.tmmu.com.cn
  • 基金资助:
    国家自然科学基金资助项目(30200249);重庆市攻关课题(2001-8-4)

The Biological Activities of Conditioned Medium Derived from Human Dermal Papilla Cells Cultured In Vitro

LUO Yang, HAO Fei, ZHONG Bai-yu, MAI Yue, JIANG Xiao-yong   

  1. Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
  • Received:2003-11-03 Online:2004-11-15 Published:2004-11-15

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摘要： 目的 研究人毛乳头细胞条件培养基的生物学活性。方法 通过体外培养低传代人毛乳头细胞,收集其基础培养基的上清液配制成条件培养基,用于培养高传代人毛乳头细胞,观察细胞形态及细胞生长曲线的变化;同时观察高传代人毛乳头细胞与低传代人毛乳头细胞共培养情况。结果 低传代条件培养基使高传代人毛乳头细胞出现了凝集生长现象,其生长曲线显著优于基础培养基培养的高传代毛乳头细胞(P<0.01),其细胞的3H鄄TdR测定值亦显著升高(P<0.01)。高传代人毛乳头细胞与低传代人毛乳头细胞共培养后出现凝集性生长趋势。结论 低传代人毛乳头细胞在培养状态下可分泌具有明确生物学活性的物质。

关键词: 毛囊, 培养基,条件性, 细胞培养

Abstract: Objective To investigate the biological activities of a conditioned medium for human dermal papilla.Methods Culture medium of the lower passage human dermal papilla cells was collected as the conditioned medium.The growth pattern and the growth curve of the higher passage human dermal papilla cells cultured with conditioned medium were observed in vitro.And the morphology of the co-culture of the higher passage human dermal papilla cells and the lower passage human dermal papilla cells was observed.Results The higher passage human dermal papilla cells,which was cultured with conditioned medium from the lower passage human dermal papilla cells,showed aggregative growth pattern.And the growth curve of the higher passage human dermal papilla cells was much better than that in the control groups (P<0.01),and the values of 3H-TdR incorporation were higher than those in the control groups (P<0.01).The co-cultured higher passage human dermal papilla cells with lower passage human dermal papilla cells showed aggregative growth pattern.Conclusion These results show that the lower passage human dermal papilla cells cultured in vitro might secrete some chemotactic factors that have some biological activities on the high passage human dermal papilla cells.

Key words: Culture media,conditioned, Cell culture, Hair Follicle