中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (10): 581-583.

• 论著 • 上一篇    下一篇

反义c-jun寡核苷酸对成纤维细胞中波紫外线辐射损伤的抑制作用

宋秀祖, 夏济平, 毕志刚   

  1. 南京医科大学第一附属医院皮肤科 210029
  • 收稿日期:2003-07-26 出版日期:2004-10-15 发布日期:2004-10-15
  • 基金资助:
    国家自然科学基金资助项目(30271195)

Inhibitory Action of Antisense c-jun oligodeoxynucleotides on Ultraviolet B Induced Fibroblast Damage

SONG Xiu-zu, XIA Ji-ping, BI Zhi-gang   

  1. Department of Dermatology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China
  • Received:2003-07-26 Online:2004-10-15 Published:2004-10-15

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摘要： 目的 探讨反义c-jun寡核苷酸(ODN)转染体外培养成纤维细胞后,对中波紫外线(U-VB)辐射诱导基质金属蛋白酶(MMP)表达的抑制作用。方法 用蛋白印迹法测定UVB辐射后,成纤维细胞原癌基因c-jun和c-fos的蛋白c-jun和c-fos的表达变化;用RT-PCR方法测定c-jun反义ODN转染后,其mRNA的表达变化。用ELISA方法测定c-jun反义ODN转染后,对UVB辐射诱导的pro-MMP-1和MMP-3的合成变化。结果 不同剂量UVB(10、20、30mJ/cm2)辐射成纤维细胞,均可诱导c-jun蛋白表达显著增加,分别为未辐射对照组的1.8、2.6、3.3倍;而c-fos的表达未见显著变化。UVB辐射还可以显著增加pro-MMP-1和MMP-3的合成。不同浓度c-jun反义ODN转染成纤维细胞后,均可以抑制UVB诱导的c-junmRNA表达;对UVB诱导的pro-MMP-1和MMP-3合成具有显著抑制作用。经统计学分析,差异有显著性(P<0.01)。结论 UVB辐射诱导的pro-MMP-1和MMP-3表达可以由c-jun介导;c-jun反义ODN可以抑制pro-MMP-1和MMP-3的合成。

关键词: 紫外线, 成纤维细胞, 基因, jun, 基因, fos, 基质金属蛋白酶

Abstract: Objective To explore the inhibitory action of antisense c-jun oligodeoxynucleotides(ODN) on matrix metalloproteinase(MMP) expression of fibroblasts induced by ultraviolet B (UVB). Methods The c-jun and c-fos protein expression induced by UVB were measured by Western blot. The mRNA expression of c-jun was determined by reverse transcriptase polymerase chain reaction (RT-PCR) after transfection with c-jun antisense ODN. The pro-MMP-1 and MMP-3 synthesis of fibroblasts was measured by ELISA after treatment with UVB and antisense c-jun ODN. Results The UVB-induced c-jun protein expression of fibroblasts increased to 1.8, 2.6, 3.3 times compared with that of non-irradiated controls,while there was no significant change in c-fos protein expression. The pro-MMP-1 and MMP-3 synthesis induced by UVB irradiation increased obviously. After transfection with different concentrations of c-jun antisense ODN, the UVB-induced c-jun mRNA expression could be significantly inhibited(P<0.01), and so was the pro-MMP-1 and MMP-3 synthesis(P<0.01). Conclusions UVB-induced pro-MMP-1 and MMP-3 expression can be mediated by c-jun, and the synthesis of pro-MMP-1 and MMP-3 may be inhibited by antisense c-jun ODN.

Key words: Ultraviolet rays, Fibroblasts, Genes, jun, Genes, fos, Matrix metalloproteinases