中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (8): 443-445.

• 论著 • 上一篇    下一篇

一株耐热红色毛癣菌临床分离株的基因型鉴定

章强强, 李民, 朱利平, 李莉, 王家俊   

  1. 上海复旦大学附属华山医院 200040
  • 收稿日期:2002-08-09 出版日期:2003-08-15 发布日期:2003-08-15

Granulomatous Skin Lesion Due to Hot-resistant Trichophyton rubrum and Its Variation in Molecular Biology

ZHANG Qiang-qiang, LI Min, ZHU Li-ping, LI Li, WANG Jia-jun   

  1. Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2002-08-09 Online:2003-08-15 Published:2003-08-15

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摘要：

目的 探讨1株引起特殊皮肤肉芽肿损害的红色毛癣菌菌株的真菌学特点及其基因序列的变化。方法 常规真菌学鉴定法和使用PCR方法测定菌株的核糖体保守区及非转录区(NTS)内trs-1区基因序列。结果 耐热菌株经真菌学鉴定及核糖体保守区基因序列测定均证实为红色毛癣菌,但其核糖体基因非转录区(NTS)内trs-1(串联重复亚单位)区基因序列测定显示有明显的不同。结论 核糖体基因NTS区内trs-1区基因序列的改变可能的解释为此株耐热红色毛癣菌为红色毛癣菌的一个同型变种,这一变种具有较强的侵袭力,能导致特殊的皮肤损害;亦或为菌株为适应外界环境条件改变发生的基因水平的变异。

关键词: 发癣菌属, 序列分析, Majocchi肉芽肿

Abstract:

Objective To investigate the mycologic features and variation in gene sequence of a hot-resistant Trichophyton rubrum which caused granulomatous skin lesion.Methods The isolate was identified by routine and polymerase chain reaction(PCR)technique.Ribosomal conserved sequence and trs-1(tandem repetitive subelement)sequence in the ribosomal non-transcribed spacer(NTS)were determined.Results The hot-resistant isolate was identified as T.rubrum by routine and PCR technique.The trs-1 sequence variation in rDNA NTS was found in the strain.Conclusion The trs-1sequence variation suggests that this strain be one of isotypic variants of T.rubrum,which has much higher pathogenicity to cause granulomatous skin lesion,or sequence variation of the strain may be the result of adapting itself to environmental change(37℃),which needs further studies.

Key words: Trichophyton, Sequence analysis, Majocchi’s granuloma