中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (4): 210-212.

• 论著 • 上一篇    下一篇

用聚合酶链反应检测结节病皮损中分支杆菌16SrRNA基因和结核杆菌DNA

蔡林, 张建中   

  1. 北京大学人民医院皮肤科 100044
  • 收稿日期:2002-06-17 出版日期:2003-04-15 发布日期:2003-04-15
  • 基金资助:
    北京大学985项目资助

Detection of Mycobacterial16S rRNA Gene and M.tuberculosis DNA in Skin Lesions of Sarcoidosis with Polymerase Chain Reaction

CAI Lin, ZHANG Jian-zhong   

  1. Department of Dermatology, People's Hospital, Peking University, Beijing 100044, China
  • Received:2002-06-17 Online:2003-04-15 Published:2003-04-15

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摘要： 目的 探讨分支杆菌感染在结节病发病中的可能作用。方法 用聚合酶链反应(PCR)检测了12例结节病患者皮损中分支杆菌16SrRNA基因片段和特异性结核分支杆菌复合物IS6110DNA片段。结果 12例结节病患者中,6例皮损中检出了分支杆菌16SrRNA基因,其中5例证实为结核分支杆菌,另1例为非结核分支杆菌。PCR扩增所得到的IS6110DNA片段经进一步测序证实。结论 分支杆菌特别是结核杆菌感染可能是结节病的病因之一。

关键词: 结节病, 分支杆菌属, 聚合酶链反应

Abstract: Objective To investigate the possible role of mycobacterial infection in the pathogenesis of sarcoidosis.Methods DNA fragments of mycobacterial 16S rRNA gene and the complex-specific insertion sequence IS6110 of M.tuberculosis were detected by polymerase chain reaction(PCR)in formalin-fixed,paraffin-embeded skin biopsy specimens from12patients with sarcoidosis.Results Mycobacterial common sequence16S rRNA gene was found in6patients,among which5were further confirmed as M.tuberculosis,the other one was atypical mycobacteria.The IS6110DNA fragment amplified by PCR was confirmed by DNA sequencing.Conclusion The above findings suggest an aetiological role of mycobacterial infection,especially M.tuberculosis,in some patients with sarcoidosis.

Key words: Sarcoidosis, Mycobacterium, Polymerase chain reaction