中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (4): 207-209.

• 论著 • 上一篇    下一篇

14种中药提取成分对酪氨酸酶活性的抑制作用

杨柳依1, 曹煜1, 魏羽佳1, 雷大卫2   

  1. 1. 贵阳医学院附属医院皮肤科 550004;
    2. 贵阳医学院生化教研室
  • 收稿日期:2002-04-04 出版日期:2003-04-15 发布日期:2003-04-15

Inhibitory Effects of Extracted Components from Chinese Medicinal Herbs on Tyrosinase

YANG Liu-yi1, CAO Yu1, WEI Yu-jia1, LEI Da-wei2   

  1. Department of Dermatology, Affiliated Hospital of Guiyang Medical College, Guiyang 550001, China
  • Received:2002-04-04 Online:2003-04-15 Published:2003-04-15

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摘要： 目的 寻找更合理的试管内分析方法研究药物对体外酪氨酸酶活性的调节,筛选对酪氨酸酶活性抑制作用显著的中药有效成分。方法 采用蘑菇酪氨酸酶多巴速率氧化法测定酪氨酸酶活性,观察14种中药有效成分在试管内对酪氨酸酶系统的调节作用。结果 10种中药提取的成分在不同浓度对酪氨酸酶活性、黑素生成量上显示了剂量依赖性抑制。其中丁香酚、姜黄油、没食子酸、丁香酚和姜黄素复合物(1:1)以及前两者与蜕皮激素(ecdysone)的复合物(1:1:1)对酪氨酸酶抑制活性与单体化合物熊果甙比较差异有显著性(P<0.05);丁香酚、姜黄油对酪氨酸酶呈竞争性抑制,没食子酸呈反竞争性抑制。结论 丁香酚、姜黄素、没食子酸和其他中药有效成分对酪氨酸酶具有体外抑制作用。

关键词: 一元酚单氧酶, 丁香酚, 姜黄素, 没食子酸, 蜕皮激素, 中草药

Abstract: Objective To develop an in vitro method to determine the effects of drugs on tyrosinase(TYR)activity and screen the inhibitory effects on TYR of 14 extracted components of Chinese medicinal herbs.Methods The activity of TYR was detected by revised Matsuda's method.Effects of14extracted components on tyrosinase system were observed in vitro.Results Ten of the 14 components showed dose-dependent down-regulation of tyrosinase activity and melanogenesis.The inhibitory activities of eugenol,curcumin and gallic acid were significantly higher than those of arbutin(P<0.05).The combinations of eugenol and curcumin(1:1),and eugenol,curcumin and ecdysone(1:1:1)had significantly higher inhibitory activities as compared to those of arbutin(P<0.05).It was indicated that eugenol and curcumin acted as competitive inhibitors while gallic acid as an opposite competitive inhibitor.Conclusion Eugenol,curcumin,gallic acid and other effective components of traditional Chinese medicines can inhibit TYR in vitro.

Key words: Monophenol monooxygenase, Eugenol, Curcumin, Gallic acid, Ecdysone, Drugs,Chinese herbal