中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (1): 38-40.

• 论著 • 上一篇    下一篇

天疱疮患者外周血单一核细胞γ干扰素、白介素10mRNA表达水平的研究

骆肖群1, 金岩1, 李锋1, 徐颂立2, 傅海军3, 翁孟武1   

  1. 1. 复旦大学附属华山医院皮肤科, 上海200040;
    2. 复旦大学生化教研室;
    3. 上海第二医科大学爱尔药物研究及临床药理中心
  • 收稿日期:2002-02-28 出版日期:2003-01-15 发布日期:2003-01-15

Expression of IFN-γmRNA and IL-10mRNA on Peripheral Blood Mmononuclear Cells of Patients With Pemphigus

LUO Xiao-qun1, JIN Yan1, LI Feng1, XU Song-li2, FU Hai-jun3, WENG Meng-wu1   

  1. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2002-02-28 Online:2003-01-15 Published:2003-01-15

PDF (PC)

102

赞

摘要： 目的 探讨天疱疮患者发病期和缓解期辅助性T细胞(Th)发病模式。方法 应用逆转录-聚合酶链反应(RT-PCR)检测20例天疱疮患者急性发病期、缓解期和20例正常对照外周血单一核细胞干扰素γ(IFN-γ)、白介素10(IL-10)、mRNA的表达水平。结果 天疱疮患者急性发病期IFN-γmRNA水平高于正常人,差异有显著性(P<0.001);缓解期IFN-γmRNA水平与正常人差异无显著性(P=0.9800)。发病期IL-10mRNA水平低于正常人,但差异无显著性(P=0.0834)。结论 天疱疮患者发病过程中有Th1/Th2细胞失平衡现象,发病期Th1型细胞因子IFN-γ可能占优势。

关键词: 天疱疮, 干扰素Ⅱ型, 白细胞介素10, RNA,信使

Abstract: Objective To probe the Th cytokine pattern in pemphigus,so as to clarify the pathogenesis of pemphigus and draw up new therapy.Methods Reverse transcription-polymerase chain reaction(RT-PCR)was used to examine the interferon gamma(IFN-γ)and interleukin 10(IL-10)mRNA expression on peripheral blood mononuclear cells(PBMCs)of 20 cases of pemphigus in acute stage,20 cases in stable stage,and 20 normal controls.Results The IFN-γmRNA expression in 20 cases of pemphigus in acute stage was higher than that of controls(P<0.001),while in stable stage there was no difference with that of controls(P=0.98).IL-10mRNA expression of pemphigus in acute stage was lower than that of controls,but the difference was not significant(P=0.0834).Conclusion IFN-γmRNA expression is predominant in acute stage of pemphigus.

Key words: Pemphigus, Interferon typeⅡ, Interleukin-10, RNA,messenger