中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (5): 396-398.

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基质金属蛋白酶10、金属蛋白酶组织抑制因子2等在皮肤鳞状细胞癌中的表达

樊翌明, 李慧忠, 吴志华, 李顺凡   

  1. 广东医学院附属医院皮肤科, 湛江524001
  • 收稿日期:2002-02-20 出版日期:2002-10-15 发布日期:2002-10-15

Expression of Matrix Metalloproteinase 10, Tissue Inhibitor of Metalloproteinase 2, Type Ⅳ Collagen and Laminin in Cutaneous Squamous Cell Carcinoma

FAN Yiming, LI Huizhong, WU Zhihua, LI Shunfan   

  1. Department of Dermatology, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, China
  • Received:2002-02-20 Online:2002-10-15 Published:2002-10-15

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摘要： 目的 了解皮肤鳞状细胞癌(简称鳞癌)中基质金属蛋白酶10(MMP-10)、金属蛋白酶组织抑制因子2(TIMP-2)、Ⅳ型胶原(ColⅣ)和层粘连蛋白(LN)的表达及其与癌分化、淋巴结转移的关系。方法 用ABC免疫组化技术观察皮肤鳞癌患者的手术切除标本48例(高分化鳞癌34例,低分化鳞癌14例),其中伴淋巴结转移者12例。结果 MMP-10在低分化鳞癌中的表达明显高于高分化组(P<0.05),但其表达在淋巴结转移组与无转移组之间的差异无显着性(P>0.05).TIMP-2、ColⅣ和LN在高分化或无淋巴结转移的鳞癌中的表达明显高于低分化或有淋巴结转移者(P<0.05-0.01).结论 皮肤鳞癌中TIMP-2、ColⅣ和LN的表达与癌分化、淋巴结转移有关,而MMP-10表达仅与癌分化有关。

关键词: 癌,鳞状细胞, 金属蛋白酶类, 金属蛋白酶2组织抑制剂, 胶原, 层粘连蛋白

Abstract: Objective To investigate the expression of matrix metalloproteinase 10(MMP-10),tissue inhibitor of metalloproteinase 2(TIMP-2),collagen typeⅣ(ColⅣ)and laminin(LN)in cutaneous squamous cell carcinoma (SCC)and the relationship between their expression and tumor differentiatio n and lymph node metastasis.Methods The expression of MMP-10,TIMP-2,ColⅣ and LN was observed by ABC immunohistochemical technique in 48 cutaneous SCCs (including 12SCCs with lymph node metastasis),among themthere were 34 highly differentiated and 14 poorly differentiated ones.Results There was a detectable staining reaction for MMP-10and TIMP-2 within cytoplasm of tumor cells and some fibroblast-like and endothelial cells surrounding neopl astic islands.The immunostaining of ColⅣ and LN was distributed along tumor nests or cells in fragmentary,disruptively linear or continuously linear patte rns.MMP-10 expression in poorly differentiated SCCs was significantly higher than that in highly differentiated ones(P<0.05),but there was no significant difference in SCCs with or without lymph node metastasis(P>0.05).Compared with the poorly different iated SCCs or SCCs with lymph node metastasis,TIMP-2,ColⅣ and LN in well differentiated SCCs or SCCs without metastasis was upregul ated(P<0.05-0.01).Conclusion Expression of TIMP-2,ColⅣ and LNrelates to the differentiation of carcinoma and lymph node metastasis,whereas MMP-10expression only correlates with the differentiation of cutaneous SCC.

Key words: Carcinoma,squamous cell, Metallop roteinases, Tissue inhibitor of metalloproteinase-2, Collagen, Laminin