中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (4): 287-289.

• 中医中药 • 上一篇    下一篇

细胞周期G1调节因子在尖锐湿疣皮损中的表达

代夫1, 郑茂荣1, 郭晋2, 谢余澄2, 刘文丽2, 吕昭萍3, 何黎3, 孙冬菊3   

  1. 1. 第二军医大学长海医院皮肤科;
    2. 云南省第二人民医院病理科;
    3. 昆明医学院第一附属医院皮肤科
  • 收稿日期:2001-07-09 出版日期:2002-08-15 发布日期:2002-08-15

Expression of Cell Cycle Related Factors Cyclin D1,CDK4,P16MTS1 and PRb in Condyloma Acuminatum

DAI Fu1, ZHENG Maorong1, GUO Jin2, XIE Yuchen2, LIU Wenli2, LÜ Zhaoping3, HE Li3, SUN Dongju3   

  1. Department of Dermatology, Chang Hai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2001-07-09 Online:2002-08-15 Published:2002-08-15

PDF

85

赞

摘要： 目的 探讨细胞周期G1调控点(checkpoint)相关调节因子细胞周期素D1(CyclinD1)、周期素依赖性蛋白激酶4(CDK4)、多重肿瘤抑制因子(P16MTS1)及视网膜母细胞瘤蛋白(PRb)在HPV6/11相关尖锐湿疣(CA)皮损中的表达和意义.方法 采用SP免疫组化染色技术检测27例经PCR检测含HPV6/11DNA的CA皮损和10例正常人皮肤(包皮)中CyclinD1、CDK4、P16MTS1及PRb的表达和分布.结果 ①正常人皮肤及CA皮损均无CyclinD1表达.②CA皮损基底细胞表达CDK4(均数2.19)较正常人皮肤(均数4.8)降低(P<0.01).③CA皮损棘细胞表达P16MTS1(均数1.33)较正常人皮肤(均数0)增强(P<0.05).④CA皮损基底细胞和棘细胞表达PRb(均数各为0.89、0.44)较正常人皮肤(均数均为0)均增强(P<0.05).结论 HPV6/11CA相关皮损的细胞周期G1调控点无明显异常,可能是这类CA皮损不易恶变的原因之一.

关键词: 细胞周期蛋白D1, 细胞周期蛋白质依赖激酶类, 蛋白质p16, 视网膜母细胞瘤蛋白质, 尖锐湿疣

Abstract: Objective To investigate the expression and im plication of cell-cycle related factors(Cyclin D1,CDK4,P16MTS1 and PRb)in condyloma acuminatum(CA).Methods The expression and distribution of cyclin D1,CDK4,P16MTS1 and PRb were detected by immunohisto chemical technique streptavidin peroxidase(SP)in 27cases of HPV6/11 positive CA that were confirmed by PCR and 10cases of normal skins(foreskins).Results ①Cyclin D1was not expressed in both normal skin and lesions of CA.②The intensity of expression of CDK4 was 2.19in the basal cells in the lesions of CA,which was significantly weaker than that in normal skin(4.8,P<0.01).③The intensity of expression of P16MTS1 was 1.33 in prickle cells in the lesio ns of CA,which was significantly stronger than that in normal skin(0,P<0.05).④The intensity of expression of PRb in both basal and prickle cells of CA were 0.89 and 0.44,respectively,which were significantly stronger than those in normal skin(0and 0,P<0.05).Conclusion There is no significant alteration in G1 check point of cell cycle in the lesions of CArelated to HPV6/11.

Key words: Condylomata acuminata, Cyclin D1, Cyclin-dependent kinases, Protein p16, Retinoblastoma protein