皮肤鳞状细胞癌中Basigin/CD147表达与肿瘤进展的相关性

中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (4): 272-275.

皮肤鳞状细胞癌中Basigin/CD147表达与肿瘤进展的相关性

陈翔¹, 金藏拓郎², 张桂英³, 肖嵘³, 颜兰香³, 文海泉³, 陈服文¹, 神崎保²

1. 中南大学湘雅医院皮肤科长沙 410008;
2. 日本鹿儿岛大学医学部皮肤科;
3. 中南大学湘雅二医院皮肤科

收稿日期:2001-10-10 出版日期:2002-08-15 发布日期:2002-08-15
基金资助:
国家自然科学基金(20040028)

The Correlation Between Basigin/CD147 Expression in Cutaneous Squamous Cell Carcinoma and Tumor Progression

CHEN Xiang¹, Takuro KANEKURA², ZHANG Guiying³, XIAO Rong³, YAN Lanxiang³, WEN Haiquan³, CHEN Fuwen¹, Tamotsu KANZAKI²

Department of Dermatology, XiangYa Hospital, Central South University, Changsha 410011, China

Received:2001-10-10 Online:2002-08-15 Published:2002-08-15

摘要/Abstract

摘要： 目的探讨Basigin/CD147在皮肤鳞状细胞癌(鳞癌)进展中的作用及其机制.方法运用免疫组化方法检测36例浸润和复发/转移情况不同的原发皮肤鳞状细胞癌标本中Basigin和基质金属蛋白酶(MMPs)的表达.运用免疫印迹和免疫荧光法观察Basigin在培养角质形成细胞和鳞状细胞癌细胞中的表达和定位.结果有浸润和伴复发/转移的原发鳞癌肿瘤细胞中Basigin、MMP-1、MMP-2和MT1-MMP的表达显着增高.肿瘤细胞周围成纤维细胞中MMP-1、MMP-2和MT1-MMP的表达亦与原发鳞癌的复发/转移密切相关.肿瘤细胞膜上Basigin的表达与成纤维细胞中MMP-1、MMP-2、MMP-3和MT1-MMP的表达间显着相关.增高表达的Basigin主要分布于鳞癌细胞膜的微绒毛上.结论鳞癌细胞膜上增高表达的Basigin/CD147可能与周围成纤维细胞作用,诱导其MMPs产生,并通过这一机制在鳞癌进展中发挥重要作用.

关键词: 癌,鳞状细胞, 金属蛋白酶类, Basigin/CD147

Abstract: Objective To investigate a possible role of basigin/CD147and a mechanism how basig in/CD147 functions in the progression of cutaneous squamous cell carcinoma (SCC).Methods Expression of basigin and matrix metalloproteinases(MMPs)was examined immunohistochemically in 36 specimens taken fromthe patie nts with SCC.Expression was compared between 7SCC in situ and 29 invasive SCC as well as 19SCC without recurrence/metastasis and 17with recurrence/metastasis.Immunoblot analysis and immunofluorescence microscopy were used to observe the expression and localization of basigin in SCC cells in vitro.Results Expression of basigin in primary SCC increased during the progression of tumor invasion.Primary SCC with recurrence/metastasis exh ibited significantly increased basigin expression compared to primary SCC without recurrence/metastasis.There were significant differences between SCCin situ and invasive SCCin the expression of MMP-1,MMP-2 and MT1-MMP in tumor cells and between SCC with and without recurrence/metastasis in the expression of MMP-1,MMP-2 and MT1-MMP in both tumor cells and fibro blasts.Significant correlation was found between basigin expression in tumor cells and MMP-1,MMP-2,MMP-3 and MT1-MMP expression in surrounding fibr oblasts.Immunoblot analysis revealed increased basigin expression in culture SCC cells.Basigin was found to localize on microvilli-like structures on cell membranes of tumor cells by immunofluorescence microscopy.Conclusions These results suggest that basigin/CD147 expressed in SCC cells might interact with surrounding fibroblasts to stimulate their MMPs expression and play an important role in the prog ression of SCC through this interaction.

Key words: Carcinoma,squamous cell, Metalloproteinases, Basigin/CD147

陈翔, 金藏拓郎, 张桂英, 肖嵘, 颜兰香, 文海泉, 陈服文, 神崎保. 皮肤鳞状细胞癌中Basigin/CD147表达与肿瘤进展的相关性[J]. 中华皮肤科杂志, 2002, 35(4): 272-275.doi:

CHEN Xiang, Takuro KANEKURA, ZHANG Guiying, XIAO Rong, YAN Lanxiang, WEN Haiquan, CHEN Fuwen, Tamotsu KANZAKI. The Correlation Between Basigin/CD147 Expression in Cutaneous Squamous Cell Carcinoma and Tumor Progression[J]. Chinese Journal of Dermatology, 2002, 35(4): 272-275.doi:

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