中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (5): 336-339.

• 论著 • 上一篇    下一篇

白念珠菌与培养人角质形成细胞的相互作用及其分子机理

冉玉平1, 坪井良治2, 李薇1, 小川秀兴2, 周光平1   

  1. 1. 四川大学华西医院皮肤性病科, 成都 610041;
    2. 日本顺天堂大学医学部皮肤科教室
  • 收稿日期:2000-02-21 出版日期:2001-10-15 发布日期:2001-10-15
  • 基金资助:
    国家自然科学基金(39770685)

Interaction between Candida albicans and Cultured Human Keratinocytes

RAN Yuping1, Tsuboi Rioji2, LI Wei1   

  1. Department of Dermatovenereology, West China Hospital, Sichuan University, Chengdu 610041, China
  • Received:2000-02-21 Online:2001-10-15 Published:2001-10-15

摘要: 目的 研究白念珠菌与培养人角质形成细胞直接作用的形态学、生物学反应及其分子机理.方法 ①将白念珠菌孢子(或煮沸灭活的白念珠菌孢子、直径3.2μm的乳胶颗粒)加入角质形成细胞培养系统中定时用扫描和透射电镜观察;②用荧光法和计数法测定白念珠菌等与角质形成细胞共同培养上清液对角质形成细胞生长的影响;③用ELISA法测定白念珠菌或乳胶颗粒与角质形成细胞共同培养上清液和角质形成细胞内液中细胞因子的含量.结果 ①发现与角质形成细胞粘附的白念珠菌随时间经过而增加,灭活的白念珠菌几乎不发生粘附,乳胶颗粒与角质形成细胞粘附后被吞噬.角质形成细胞伸出很多纤维样结构与菌体或乳胶颗粒粘附;②白念珠菌与角质形成细胞共同培养液在50%浓度下可显著促进角质形成细胞增殖,灭活的白念珠菌或乳胶颗粒与角质形成细胞共同培养液对角质形成细胞增殖有一定的促进作用;③白介素1α在白念珠菌与角质形成细胞共同培养上清液中增高,但细胞内液中下降,转化生长因子-α和碱性成纤维细胞生长因子在上清液及细胞内液中水平均增高.结论 角质形成细胞具有非特异性吞噬功能.白念珠菌与角质形成细胞作用可刺激角质形成细胞释放细胞因子,引起炎症反应和细胞生长.

关键词: 念珠菌,白色, 角蛋白细胞, 相互作用

Abstract: Objective To study the morphological,biologic response of cultured human keratin ocytes to Candida albicans and the molecular mechanism.Methods ① A yeast form of C. albicans was added to a monolayer of keratinocytes.The adherent organisms were observed by scanning and transmission electron microscope at different incubation periods. Boiled C. albicans and latex beads of a similar size(3.2μm) were also inoculated for comparison.② The effect of supernatant from the coculture of keratinocytes with intact C. albicans on keratinocyte growth was measured by the fluorescence intensity and cell count.③ Keratinocytes were incubated with C. albicans or latex beads,and the level of cytok ine in the keratinocytes and supernatant were measured with enzymelinked im munosorbent assay kits.Results ① The number of adherent C. albicans increased with the lapse of time,while boiled C. albicans did not adhere at all.Many latex beads adhered to the keratinocytes,and were then easily phagocytised.Fibril-like structures stretched from the keratinocytes adhered to the organisms or latex beads.② The conditioned medium of 50% concen tration significantly promote cell growth,while that with boiled C. albicans or latex beads moderately stimulated keratinocyte growth.③ Keratinocytes treated with intact C. albicans had significantly higher level of IL-1α in the supernatant but lower in the cell extract.Both TGF-α and bFGF increased either in the media or in the extract.Conclusion These Results suggest that keratinocytes have nonspecific phagocytic activity. C. albicans are able to adhere to keratinocytes and stimulate the release of various cytokines from keratinocytes,which may induce an inflammatory reaction and cell growth.

Key words: Interactions, Candida albicans, Keratinocytes