中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (5): 340-342.

• 论著 • 上一篇    下一篇

白念珠菌烯醇化酶的抗原特异性研究

唐宁枫1, 焦瑞清2, 吴绍熙1, 郭宁如1, 秦浚川2   

  1. 1. 中国医学科学院、中国协和医科大学真菌中心, 南京 210042;
    2. 南京大学医药生物技术国家重点实验室
  • 收稿日期:2001-01-03 出版日期:2001-10-15 发布日期:2001-10-15
  • 基金资助:
    卫生部重点项目(97010211);南京大学医药生物技术国家重点实验室开放基金资助(XW199902)

Study on Antigenic Specificity of Candida albicans Enolase

TANG Ningfeng1, JIAO Ruiqing2, WU Shaoxi1   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042, China
  • Received:2001-01-03 Online:2001-10-15 Published:2001-10-15

PDF (PC)

88

赞

摘要： 目的 探讨白念珠菌烯醇化酶的抗原特异性.方法和结果 ①免疫印迹显示烯醇化酶是白念珠菌非糖基化蛋白中可以和感染兔血清发生反应的主要蛋白质.②以纯化的白念珠菌烯醇化酶免疫家兔获得最高效价为1:6400的多克隆抗体(ELISA法测定).③与另外3种多克隆抗体相比,抗烯醇化酶抗体在检测白念珠菌菌体抗原时显示出较高的特异性.结论 白念珠菌烯醇化酶是一种值得进一步研究的特异性抗原.

关键词: 烯醇化酶, 抗原, 念珠菌,白色

Abstract: Objective To evaluate the antigenic specificity of the enolase.Methods and Results The Results included:(1) Western blot analysis demonstrated that enolase was a main protein in the nonglycoproteins of C. albicans which could react with the serum of C. albicans infected rabbit.(2) The purified enolase was used to immunize rabbit for producing antienolase serum,and the highest titer of polyclonal antibody against enolase was up to 1:6400 with enzymelinked immunoadsorbent assay(ELISA).(3)Compared with three other polyclonal antibodies,antienola seantibody showed high specificity in detecting C. albicans antigen.Conclusion Enolase as a specific antigen,is worthy of further investigation in C. albicans cell.

Key words: Candida albicans, Antigens, Enolase