中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (4): 266-267.

• 论著 • 上一篇    下一篇

他克莫司对朗格汉斯细胞迁移能力的影响

顾军, 唐玲, 毕新岭, 王志东, 王英   

  1. 上海第二军医大学附属长海医院皮肤科 200433
  • 收稿日期:2000-10-08 出版日期:2001-08-15 发布日期:2001-08-15

The Effect of Tacrolimus on Langerhans Cell Migration

GU Jun, TANG Ling, BI Xinling   

  1. Department of Dermatology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China
  • Received:2000-10-08 Online:2001-08-15 Published:2001-08-15

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摘要： 目的 观察他克莫司对朗格汉斯细胞(LC)迁移能力的影响,为明确他克莫司在治疗疾病中的作用途径打下基础。方法 在不同时间段内将不同浓度的他克莫司腹腔注射C57BL/6小鼠,再用异硫氰酸荧光素(FITC)刺激小鼠耳背部皮肤,12h后通过流式细胞仪检测小鼠耳背部淋巴结内摄取抗原的LC数量。通过免疫组化法检测抗原致敏处LC的数量。结果 他克莫司腹腔注射过的小鼠耳背皮肤受到FITC刺激后,其皮肤局部摄取抗原的LC迁移至局部淋巴结的数量明显减少,尤其在FITC刺激前12h注射他克莫司的小鼠皮肤局部LC的迁移能力受到显著抑制,在注射高剂量他克莫司组与低剂量组之间存在明显差异。而同时他克莫司腹腔注射过的小鼠,其耳背部受到FITC刺激后的皮肤局部LC的数量高于未注射组。结论 他克莫司作为一种免疫抑制剂,其发挥治疗作用途径有可能是通过抑制LC的迁移能力,从而阻止或减弱了某些免疫反应。

关键词: 他克莫司, 朗格汉斯细胞, 趋化性

Abstract: Objective To investigate the effect of tacrolimus on Langerhans cell migration in order to understand the therapeutic mechanism of tacrolimus. Methods C57BL/6 mouse injected with different doses of tacrolimus was stimulated by FITC at ear back. After twelve hours, the number of Langerhans cells which took antigen and migrated to lymph nodes was measured by flow cytometry. The number of Langerhans cells which took antigen and resided in epidermis was examined by immunohistochemical staining. Results After injection with tacrolimus, the number of Langerhans cells which took antigen and migrated to lymph nodes was reduced, especially at 12 hours before stimulation by FITC. The reduction was significantly different between the high dose and low dose injection groups. However,the number of Langerhans cells residing in epidermis was significantly higher in tacrolimus injection group than that in the control. Conclusions Tacrolimus, a new kind of immunosuppressive drug,may probably suppress Langerhans cell migration and therefore inhibit immune response in some diseases.

Key words: Tacrolimus, Langerhans cells, Chemotaxis