中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (4): 263-265.

• 论著 • 上一篇    下一篇

重组腺病毒载体转染CTLA4Ig基因在毛乳头细胞中的表达

程波1, 刘荣卿2, 伍津津3, 李家锋1, 何勤1, 钟白玉2   

  1. 1. 贵州省贵阳医学院附属医院皮肤科 550004;
    2. 第三军医大学西南医院皮肤科;
    3. 第三军医大学大坪医院皮肤科
  • 收稿日期:2000-11-27 出版日期:2001-08-15 发布日期:2001-08-15
  • 基金资助:
    国家自然科学基金资助课题(30070375)

Expression of CTLA4Ig Gene in Dermal Papilla Cells Transferred with Recombinant Adenovirus Vector

CHENG Bo1, LIU Rongqing2, WU Jinjin3   

  1. Department of Dermatology, Affiliated Hospital, Guiyang Medical College, Guiyang 550004, China
  • Received:2000-11-27 Online:2001-08-15 Published:2001-08-15

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摘要： 目的 探讨免疫耐受基因(CTLA4Ig)在毛乳头细胞中的表达,为毛乳头细胞移植后产生免疫耐受提供理论和实验依据。方法 通过构建的重组腺病毒载体将免疫耐受基因(CTLA4Ig)cDNA导入培养的毛乳头细胞中,并将体外基因转染的毛乳头细胞移植到小鼠体内,通过组织学和免疫组化检测目的基因在体内和体外的表达。结果 CTLA4Ig蛋白在基因转染6h后即在细胞浆中表达,随着转染时间的延长其表达逐渐增加;基因转染的毛乳头细胞移植到小鼠体内,目的基因在24h开始表达,能持续表达2周;移植细胞未受到免疫排斥。结论 带有CTLA4Ig基因的毛乳头细胞在体外和小鼠体内能较长时间存活并表达CTLA4Ig。

关键词: 毛囊, CTLA4, 遗传载体, 基因转移

Abstract: Objective To study CTLA4Ig gene expressed in dermal papilla cells and to provide evidence for immune tolerance after dermal papilla cells transplantation. Methods CTLA4Ig cDNA was transferred into dermal papilla cells by recombinant adenovirus vector, and the dermal papilla cells containing CTLA4Ig gene were transplanted into mice skin. The target gene expression was detected by histological and immunohistochemistry technique. Results CTLA4Ig protein was expressed in plasma 6 hours after gene transfection and increased gradually. When the transferred papilla cells were transplanted into mice skin the gene began to express in 24 hours and lasted for 2 weeks. No rejection was observed. Conclusion Dermal papilla cells containing CTLA4Ig gene can survive in vitro and in vivo and express CTLA4Ig for a long time.

Key words: Gene transfer, Hair follicle, CTLA4Ig, Genetic vectors