中华皮肤科杂志 ›› 2001, Vol. 30 ›› Issue (1): 15-17.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者T细胞克隆性的分析

陈红清, 徐文严   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京 210042
  • 收稿日期:2000-02-14 出版日期:2001-02-15 发布日期:2001-02-15
  • 基金资助:
    中华医学会皮肤科学会基金

Analysis of T Cell Clonality in Patients with Systemic Lupus Erythematosus

CHEN Hongqing, XU Wenyan   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:2000-02-14 Online:2001-02-15 Published:2001-02-15

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摘要： 目的 研究系统性红斑狼疮(SLE)患者T细胞的克隆性.方法 用逆转录－聚合酶链反应和基因扫描技术分析了6例活动性SLE患者外周血淋巴细胞的T细胞克隆性.结果 2例SLE患者的Vβ产物来自多克隆T细胞,另4例患者的Vβ9、Vβ2、Vβ1、Vβ2产物均来自寡克隆T细胞.结论 SLE患者T细胞存在着克隆性增殖,并有可能是特定的自身抗原所引起的一种特异性应答.

关键词: 红斑狼疮,系统性, 受体,T细胞

Abstract: Objective To investigate the T cell clonality in patients with systemic lupus erythematosus (SLE). Methods T cell clonality in peripheral blood lymphocytes from six patients with active SLE was analyzed using reverse transcriptase-polymerase chain reaction (RT-PCR)and GeneScan technique. Results T cell receptor (TCR)Vβ PCR products from two patients were derived from polyclonal T cells,however,TCR Vβ 9,Vβ 2,Vβ 1,Vβ 2 products from the other four patients were derived from oligoclonal T cells respectively. Conclusion T cells from patients with SLE demonstrated a striking oligoclnality and their clonal expansion may be a specific response driven by autoantigens.

Key words: Lupus erythematosus,systemic, Receptor,T-cell