中华皮肤科杂志 ›› 2001, Vol. 30 ›› Issue (1): 18-21.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者外周血单一核细胞NF-κB信号通路活化检测

许韩师, 叶任高, 孙林, 杨琼琼, 曾丽霞, 李志坚, 李幼姬   

  1. 中山医科大学附属第一医院肾内科, 广州 510080
  • 收稿日期:2000-11-03 出版日期:2001-02-15 发布日期:2001-02-15
  • 基金资助:
    中山医科大学 211工程重点项目资助课题

Activation of NF－κ B Signaling Pathway in Peripheral Blood Mononuclear Cells of Patients With Systemic Lupus Erythematosus

XU Hanshi, YE Rengao, SUN Lin   

  1. First Affiliated Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou 510080
  • Received:2000-11-03 Online:2001-02-15 Published:2001-02-15

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摘要： 目的 检测系统性红斑狼疮(SLE)患者外周血单一核细胞(PBMC)NF－κB信号通路活化情况,并探讨其临床意义.方法 NF－κB活性检测采用电泳迁移率改变试验,IκBα蛋白及其磷酸化产物采用蛋白印迹,抗dsDNA抗体、IgG、IgM测定采用ELISA方法.结果 SLE患者PBMCNF－κB活性显著高于正常对照组(P<0.05),活动期明显高于缓解期(P<0.05),SLE组IκBα蛋白表达显著低于正常对照组(P<0.05),活动期患者低于缓解期(P<0.05).SLE组IκBα磷酸化产物显著高于正常对照组(P<0.05),活动期患者高于缓解期(P<0.05).NF－κB活性和IκBα磷酸化产物均与抗dsDNA抗体、IgG和SLE疾病活动指数呈正相关,而与IgM无相关关系.结论 SLE患者PBMC存在NF－κB信号通路异常活化,且与抗dsDNA抗体、IgG分泌密切相关.检测SLEPBMC内NF－κB信号通路蛋白表达可能有助于SLE疾病活动的判断.

关键词: 红斑狼疮,系统性, 白细胞,单核, NF-κB, IκBα

Abstract: Objective To detect the activation of NF－κ B signaling pathway in peripheral blood mononuclear cells(PBMCs)of patients with systemic lupus erythematosus(SLE)and investigate its clinical significance. Methods Activation of NF－κ B was detected by electrophoretic mobility shift assay(EMSA),the expression of Iκ Bα protein and its phosphorylated products were detected by Western blot. Anti-dsDNA antibody,IgG and IgM in supernatant of PBMC culture were tested by ELISA. Results (1)Elevated activation of NF－κ B in PBMC of SLE patients was found when compared with the controls(P<0.05),and activation of NF－κ B in active SLE group was higher than that of inactive SLE group(P<0.05). (2)The expression of Iκ Bα in SLE group was lower than that of the control group(P<0.05),and Iκ Bα in active SLE group was lower than that of inactive SLE group(P<0.05).(3)Iκ Bα phosphorylated product in SLE group was higher than that in the control group,and the product in the active SLE group was increased compared with the inactive SLE group.(4)The activation of NF－κ B and Iκ Bα phosphorylated product had a positive correlation with the titer of anti-dsDNA antibody,IgG in the supernatant of PBMC culture and SLEDAI,respectively. Conclusions There are abnormal activation of NF－κ B signaling pathway in SLE. Detection of activation of NF－κ B signaling pathway in PBMC may be used as a predicator of SLE disease activity.

Key words: Lupus erythematosus,systemic, Leukocytes,mononuclear, NF-kappa B, I kappa Bα