中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (4): 259-260.

• 论著 • 上一篇    下一篇

瘢痕疙瘩组织中内皮素-1及碱性成纤维细胞生长因子mRNA的表达

阎国富1, 何威1, 刘荣卿2   

  1. 1. 第三军医大学附属新桥医院皮肤科 重庆 400037;
    2. 第三军医大学附属西南医院皮肤科
  • 收稿日期:1999-08-02 出版日期:2000-08-15 发布日期:2000-08-15
  • 基金资助:
    国家自然科学基金资助课题(39600154)

Expression of Endothelin-1 and bFGF mRNA in Keloid Tissues

YAN Guofu1, HE Wei1, LIU Rongqing2   

  1. Department of Dermatology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037
  • Received:1999-08-02 Online:2000-08-15 Published:2000-08-15

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摘要： 目的 了解内皮素-1和碱性成纤维细胞生长因子(bFGF)在瘢痕疙瘩组织的表达情况。方法 组织活检标本分为3组,瘢痕疙瘩、萎缩性瘢痕和正常皮肤。用地高辛标记的cDNA探针,采取冰冻切片原位杂交的方法检测内皮素-1和bFGF mRNA的表达。结果 瘢痕疙瘩真皮组织内内皮素-1 mRNA的表达明显强于萎缩性瘢痕和对照组。阳性染色主要位于真皮浅层血管和部分真皮成纤维细胞。瘢痕疙瘩组6/8例真皮成纤维细胞内皮素-1 mRNA阳性,且呈弥漫性分布;萎缩性瘢痕和正常皮肤真皮成纤维细胞内皮素-1mRNA无阳性着色。结论 内皮素-1和bFGFmRNA在瘢痕疙瘩组织中的过度表达提示内皮素-1和bFGF可能在瘢痕疙瘩的形成中起一定作用。

关键词: 瘢痕疙瘩, 内皮缩血管肽-1, 成纤维细胞生长因子,碱性

Abstract: Objective To determine the expression of endothelin-1(ET-1) and basic fibroblast growth factor(bFGF) mRNA in keloid lesion. Methods Skin biopsies were obtained from patients with keloid, normal scar and healthy controls. The expression of ET-1 and bFGF mRNA was evaluated by in situ hybridization with Dig labelled cDNA probe. Results Dermal ET-1 and bFGF mRNA staining was more intensive in keloid lesion than that in scar and control tissue. Increased ET-1 staining was mainly observed in the superficial dermal vessels and dermal fibroblasts. ET-1 positive fibroblasts were found in 6 of 8 patients with keloid and diffusely distributed. Conclusion The overexpression of ET-1 and bFGF in keloid indicates that ET-1 and bFGF may play a role in the formation of keloid.

Key words: Keloid, Endothelin-1, Fibroblast growth factor,basic