中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (4): 238-239.

• 论著 • 上一篇    下一篇

热休克蛋白70在皮肤肿瘤中的表达及与增殖细胞核抗原、p53蛋白表达的相关性研究

宋珺辉, 易传勋   

  1. 同济医科大学附属协和医院整形外科 武汉 430022
  • 收稿日期:1999-07-26 出版日期:2000-08-15 发布日期:2000-08-15

Study on Heat Shock Protein 70 Expression in Skin Carcinomas and Its Relationship with PCNA and p53 Protein

SONG Junhui, YI Chuanxun   

  1. Department of Burns and Plastic Surgery, Affiliated Hospital of Nanjing Railway College, Nanjing 210009
  • Received:1999-07-26 Online:2000-08-15 Published:2000-08-15

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摘要： 目的 研究热休克蛋白70在皮肤癌中的表达水平和分布特点及与增殖细胞核抗原(PCNA)、p53蛋白表达的相关性。方法 应用免疫组化方法(SABC),用特异性热休克蛋白70单抗、p53蛋白单抗、PCNA单抗检测热休克蛋白70、PCNA、p53蛋白在皮肤良恶性病变和正常皮肤组织中的表达和分布。结果 热休克蛋白70在鳞状细胞癌、基底细胞癌癌细胞核内有异常增高表达,其中鳞状细胞癌、基底细胞癌中阳性与阴性标本的PC10指数差异有显著性,而p53蛋白阳性率未见明显差异。鳞状细胞癌中热休克蛋白70表达在Ⅰ～Ⅱ和Ⅳ级间差异有显著性。结论 热休克蛋白70在皮肤癌中存在异常增高的核表达,并与细胞的增殖程度和病理分级有关,提示其可能参与肿瘤生长,其在皮肤肿瘤中的诊断价值和与临床预后的关系需要进一步研究。

关键词: 热休克蛋白质类, 增殖细胞核抗原, 蛋白质p53, 皮肤肿瘤

Abstract: Objective To study heat shock protein (HSP)70 expression in skin carcinomas and its relationship with proliferating cell nuclear antigen (PCNA) and p53 protein. Methods The expression of HSP70, p53 protein, and PCNA was determined by an immunohistochemical technique (SABC) using specific antibodies against HSP 70, p53 protein and PCNA in a variety of benign and malignant skin tissues. Results The overexpression of HSP 70 was found in 60% of squamous cell carcinoma (SCC) and 25% of basal cell carcinoma (BCC). HSP 70 was located mainly in the nuclei of tumor cells. There was a significant difference of PC 10 indexes, which represent the positive rates of PCNA, between HSP 70 positive and negative tissues in SCC and BCC. In view of p53 protein, no significant difference was observed between HSP 70 positive and negative tissues in those tumors. The differences of HSP 70 expression were significant between gradeⅠ～Ⅱ and gradeⅣ of SCC. Conclusion The results demonstrate that HSP 70 is associated with cellular proliferation and pathological grading of skin cancers. It is suggested that HSP 70 may be involved in the progression of tumor cells. Further research is needed to elucidate the value of diagnosis and prognosis of HSP 70 in skin tumors.

Key words: Heat-shock proteins, Proliferating cell nuclear antigen, Protein p53, Skin neoplasms