中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (4): 240-242.

• 论著 • 上一篇    下一篇

16型人乳头瘤病毒E6/E7诱导角质形成细胞表达热休克蛋白70

廖文俊1, 叶苓2, 刘彦仿1, 刘玉峰1, 王春梅3   

  1. 1. 第四军医大学西京医院皮肤科, 西安 710032;
    2. 第四军医大学基础部病理学教研室, 西安 710032;
    3. 第四军医大学电镜室
  • 收稿日期:1998-08-11 修回日期:1998-12-18 出版日期:1999-08-15 发布日期:1999-08-15
  • 基金资助:
    国家自然科学基金39570656

Expression of 70-kD Heat Shock Protein in Keratinocytes Induced by Human Papillomavirus 16 E6/E7

LIAO Wenjun1, YE Ling2, LIU Yanfang1   

  1. Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xian 710032
  • Received:1998-08-11 Revised:1998-12-18 Online:1999-08-15 Published:1999-08-15

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摘要： 目的 了解16型人乳头瘤病毒(HPV16)E6/E7、HSP70与角质形成细胞永生化及转化之间的关系.方法 Lipofectamine介导法进行转染,以G418筛选阳性克隆,免疫印迹分析及免疫荧光双标记染色结合激光扫描共聚焦显微镜对HSP70和HPV16E6/E7在转染角质形成细胞中的表达进行了观察.结果 免疫印迹分析显示正常角质形成细胞和转染角质形成细胞均在相对分子质量70000处出现一条染色带,但转染细胞的着色深度明显强于正常细胞;激光扫描共聚焦显微镜观察也发现转染细胞中HSP70的表达明显增高,HSP70与HPV16E6/E7有共定位.结论 HPV16E6/E7可以诱导角质形成细胞中HSP70的高表达,这种高表达可能在角质形成细胞永生化甚至转化过程中具有重要作用.

关键词: 乳头瘤病毒,人, 热休克蛋白类, 角蛋白细胞, 转染

Abstract: Objective To investigate the relation between human papillomavirus 16 E6/E7 (HPV16 E6/E7),70 000 heat shock protein (HSP70) and immortalization and transformation of keratinocytes. Methods Keratinocytes were transfected with lipofectamine and positive clones were selected by G418. The expressions of HSP70 and HPV16 E6/E7 by transfected keratinocytes were analysed by means of Western blot,double labelling immunofluorescent staining and laser-scanning confocal microscopy techniques. Results Results of Western blot analysis showed a specific band exhibiting a molecular weight of 70000 in both normal keratinocytes and HPV16 E6/E7 transfected keratinocytes, but the staining was more intensive in transfected keratinocytes than in normal ones. The co-location of HPV16 E6/E7 and HSP70 in transfected keratinocytes were observed by laser-scanning confocal microscopy. Conclusion The high expression of HSP70 in transfected keratinocytes may be induced by HPV16 E6/E7 which suggests that HSP70 might play an important role in immortalization and even transformation of keratinocytes.

Key words: Papillomavirus, human, Heat-shock proteins, Keratinocytes, Transfection