中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (4): 237-239.

• 论著 • 上一篇    下一篇

天疱疮患者血清白介素-6、肿瘤坏死因子-α的检测分析

周淑华1, 林麟1, 靳培英1, 王海燕2, 胡涛2, 高美华2, 王峰来1, 桑红桂1   

  1. 1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京 210042;
    2. 山东滨州医学院免疫学研究室
  • 收稿日期:1998-10-26 修回日期:1999-01-08 出版日期:1999-08-15 发布日期:1999-08-15

Interleukin-6 and Tumor Necrosis Factor-αin Sera of Patients with Pemphigus

ZHOU Shuhua1, LIN Lin1, JIN Peiying1   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:1998-10-26 Revised:1999-01-08 Online:1999-08-15 Published:1999-08-15

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摘要： 目的 探讨白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)在天疱疮发病机理中的作用以及患者血清中这2种细胞因子水平与天疱疮病情的关系.方法 用双抗体夹心ELISA法检测了26例寻常型天疱疮(PV),14例红斑型天疱疮(PE)患者治疗前、8例经有效治疗皮损全部消退的PV患者及23例正常人血清中IL-6、TNF-α水平,并测定了4例PV患者治疗前疱液中IL-6、TNF-α水平.结果 天疱疮患者治疗前血清中IL-6、TNF-α水平显着高于健康对照组(P<0.01);2种细胞因子水平均与皮损面积呈正相关(P<0.01);随皮损消退其水平降低(P<0.05).4例PV患者疱液中IL-6、TNF-α水平均高于相应血清中水平.结论 天疱疮患者血清中IL-6、TNF-α水平与天疱疮病情活动性相关.IL-6、TNF-α可能参与了天疱疮的发病.

关键词: 天疱疮, 白细胞介素-6, 肿瘤坏死因子

Abstract: Objective To elucidate the role of interleukin -6 (IL-6) and tumor necrosis factor -α(TNF-α) in the pathogenesis of pemphigus, and to find out the relationship between the two cytokines and the activity of the disease. Methods The serum levels of IL-6, TNF αwere determined by double antibody sandwich enzyme liked immuno sorbant assay (ELISA) in 26 patients with pemphigus vulgaris (PV) and 14 patients with pemphigus erythematosus (PE) prior to treatment, in 8 PV patients after complete clearing of the skin lesions and in 23 normal controls. The levels of IL-6 and TNF-αwere also tested in the blister fluid obtained from 4 PV patients at the same time when the serum samples were taken. Results The results showed that the serum levels of IL-6 and TNF αwere significantly higher in patients with pemphigus than that in normal controls (P<0.01).A significant correlation was found between the serum levels of IL-6 and TNF αand the total area of lesions for each patient (P<0.01). The serum levels of the two cytokines declined as the skin lesion had completely disappeared(P<0.05). The levels of both IL-6 and TNF αin the blister fluid of 4 PV patients were higher than that in the serum samples obtained at the same time. Conclusion The results indicate that there is a relationship between the serum levels of IL-6 and TNF-αand the activity of pemphigus. The results also suggest that IL-6 and TNF-αplay an important role in the pathogenesis of pemphigus.

Key words: Pemphigus, Interleukin -6, Tumor necrosis factor