中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (3): 171-173.

• 病例报告 • 上一篇    下一篇

分段毛囊上皮细胞增生能力比较

伍津津, 刘荣卿, 唐书谦, 钟白玉, 叶庆佾   

  1. 第三军医大学西南医院皮肤科, 重庆400042
  • 收稿日期:1998-06-16 修回日期:1999-01-18 出版日期:1999-06-15 发布日期:1999-06-15

Comparison of the Proliferative Potential of Keratinocytes from Different Fragment of Hair Follicle

WU Jinjin, LIU Rongqin, YIE Qinyi   

  1. Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing 400038
  • Received:1998-06-16 Revised:1999-01-18 Online:1999-06-15 Published:1999-06-15

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摘要： 目的 阐明分段毛囊上皮细胞之间的生物学特性和毛囊干细胞的定位。方法 将毛囊上皮细胞分成上段、下段和球部,分别培养于人成纤维细胞滋养层上,然后传代,或在成纤维细胞胶原凝胶上行器官型培养。结果 上段细胞传代次数最多,形成的克隆数最多,细胞生长活力最强;而球部细胞的生长能力最差,下段细胞有少数能形成进行性生长克隆。在间质细胞胶原凝胶上上段细胞形成了层次结构清楚的表皮,下段细胞次之,球部细胞较差。结论 毛囊上段细胞的增生能力最强,分化能力最好,支持毛囊干细胞定位于隆突部的假说。

关键词: 毛囊, 干细胞

Abstract: Objective To investigate the biological characteristics of epithelial cells from different segments of hair follicle and localization of follicular stem cells. Methods The epithelial cells of hair follicles were cut into three parts:upper segment, lower segment and bulb, they were dispersed to single cells by 0.125% trypsin and 0.02% EDTA, then cultured respectively on a feeder cell layer of human dermal fibroblasts, and passaged or organotypically cultured on collagen gel of fibroblasts. Results The keratinocytes from upper segment grew best, with the most numerous colonies and passage-times. The bulb cells grew worst. A few of keratinocytes from lower-segment showed continuing growth colonies. When cocultured on collagen gel of fibroblasts, the keratinocytes from upper-segment formed a piece of well differentiated epidermis. Conclusion The keratinocytes from the upper-segment of hair follicle have the strongest proliferative potntial and best differentiation ability which support the hypothesis that the steem cells of follicle are localized in the bulge area.

Key words: Hair follicle, Stem cell